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优势特色（Features）
- Designed under ISO 9001:2015 and ISO 13485:2016
- Manufactured and QC tested under a GMP compliance factory
- FDA DMF filed
- Animal-Free materials
- Beta-lactam materials free
- Batch-to-batch consistency
- Stringent quality control tests
- No animal derived peptone and lactose used in production process
产品描述（Product Details）
GMP GENPower™ NLS-Cas9 Nuclease is a recombinant Streptococcus pyogenes Cas9 protein purified from Escherichia coli for CRISPR-based genome editing. The introduction of nuclear localization signals (NLS) can help Cas9 enter the nucleus, increasing the chance of genomic DNA cleavage.
应用说明（Application）
• Genetic modification of cells and gene therapy drugs (T cell, hematopoietic stem cell)
• High specificity detection of pathogens
浓度（Concentration）
10 mg/mL
纯度（Purity）
>95% as determined by SDS-PAGE.
>95% as determined by SEC-HPLC.
宿主蛋白残留（Host Cell Protein）
≤10 ng/mg of protein tested by ELISA.
宿主核酸残留（Host Cell DNA）
≤1 ng/mg of protein tested by qPCR.
无菌（Sterility）
The sterility testing was performed by membrane filtration method described in CP<1101>, USP<71> and Eur. Ph. 2.6.1.
内毒素（Endotoxin）
Less than 10 EU/mg by the LAL method.
制剂（Formulation）
Supplied as 0.2 μm filtered solution in 20 mM Tris, 300 mM NaCl, 0.1 mM EDTA, 1 mM TCEP, pH7.5.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
This product is stable after storage at:
- The product MUST be stored at -20°C or lower upon receipt;
- -20°C for 5 years under sterile conditions.
产品数据图
电泳（SDS-PAGE）
The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-HPLC
The purity of GMP GENPower™ NLS-Cas9 Nuclease (Cat. No. GMP-CA9S18) was greater than 95% as determined by SEC-HPLC.
活性（Bioactivity）
Different amounts of Cas9 were incubated with the same amount of excess gRNA and plasmid for 60 minutes at 37°C. When using 400-200 ng Acro Cas9, the cutting efficiency is greater than 90% (QC tested). In comparison, when using a 200 ng Competitor T, the cutting efficiency is only about 50%.
The TCR knockout efficiency with GMP GENPower™ NLS-Cas9 Nuclease in human primary T cells, GMP GENPower™ NLS-Cas9 Nuclease achieved over 95% knockout efficiency.
The cleavage efficiency in HEK293 cell 72 hours after electroporation of GMP GENPower™ NLS-Cas9 Nuclease RNP.
The cleavage efficiency in iPSC 72 hours after electroporation of GMP GENPower™ NLS-Cas9 Nuclease RNP.
The knockout efficiency for B2M in primary T cell was measured by Flow Cytometry.
The knockout efficiency for TRAC in Jurkat cell was measured by Flow Cytometry.
The knockout efficiency for B2M in Jurkat cell was measured by Flow Cytometry.
稳定性（Stability）
The bioactivity based assay shows batch-to-batch consistency between Acro's GMP and PG Cas9.
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