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应用说明（Application）
The Human Th1/Th2 Cytokine Kit can be used to quantitatively measure Interleukin-2 (IL-2), Interleukin-4 (IL-4), Interleukin-6 (IL-6), Interleukin-10 (IL-10), Tumor Necrosis Factor-α (TNF-α), and Interferon-γ (IFN-γ) protein levels in a single sample.The kit performance has been optimized for analysis of specific cytokines in tissue culture supernatants, EDTA plasma, and serum samples.
仅供研究使用。
您点击这可获取Application Note"使用ACRO Human Th1/Th2 Cytokine Kit检测PBMC被刺激前后细胞因子释放的研究"。
背景（Background）
T helper cells (Th) play a vital role in the adaptive immune system by suppressing or regulating immune responses. After Th actived, it will differentiate into T-helper 1 (Th1) or T-helper 2 (Th2) effector cells. These two subsets are characterized by their distinct cytokine expression pattern and the immune function they mediate. Th1 and Th2 cells each produce a specialized combination of cytokines that depends on type of pathogen that the helper T cell has recognized-some cytokines are more effective than others in the process of eliminating certain invaders. In general, TH1 cytokines play the role of immune killing, while Th2 cytokines play the role of immune protection. Th1/Th2 paradigm provides rationale for developing new vaccines against infectious diseases and novel strategies for the therapy of allergic and autoimmune disorders.
原理（Assay Principles）
Human Th1/Th2 Cytokine 6-plex panel is a multiplex bead-based indirect immunofluorescence assay, utilizing flow cytometry for data acquisition and classification. The beads conjugated with specific antibody, target protein (IL-2/4/6/10, TNF-α, IFN-γ) in specimens and PE-conjugated detection antibody form a sandwiches complex as bead-analyte-detection antibody. The intensity of PE fluorescence signal in proportion to the target protein in specimens, are assessed by flow cytometry.The present assay has been optimized for quntitatively analysis for the IL-2/4/6/10, TNF-α, IFN-γ in cell culture supernatant, plasma, and serum samples.
产品描述（Product Details）
Assay Type FACS Analyte IFN-γ, TNF-α, IL-10, IL-6, IL-4, IL-2 Format 96T Reactivity Human Regulatory Status RUO Sensitivity 1.77pg/ml (IFN-γ), 0.76pg/ml (TNF-α), 0.27pg/ml (IL-10), 0.78pg/ml ( IL-6), 0.65pg/ml (IL-4), 0.70pg/ml (IL-2) Standard Curve Range 9.8 ~ 10000pg/mL Assay Time 3 hr Suitable Sample Type human serum, human plasma, cell culture supernatants Sample volume 10 uL 组分（Materials Provided）
请点击这里以获取更多灵活的选择ID Components Size FCM05-C01 Calibrator 5ug（for each）×2 FCM05-C02A Human IL-2 Capture Beads 96 tests FCM05-C02B Human IL-4 Capture Beads 96 tests FCM05-C02C Human IL-6 Capture Beads 96 tests FCM05-C02D Human IL-10 Capture Beads 96 tests FCM05-C02E Human TNF-α Capture Beads 96 tests FCM05-C02F Human IFN-γ Capture Beads 96 tests FCM05-C03 2 × Assay Buffer 40 mL FCM05-C04 10 × Wash Buffer 10 mL FCM05-C05 Detection antibody 0.5 mL FCM05-C06 96-well V-bottom plate 1 plate FCM05-C07 96-well Sealing film 2 pieces FCM05-C08 APC Positive Control 0.5 mL FCM05-C09 PE Positive Control 0.5 mL 存储（Storage）
The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
质量管理控制体系（QMS）
产品数据图
流式数据 -FACS Data
Please refer to DS document for the assay protocol.
A representative scatter plot showing single bead population in P1 gate (left), the beads conjugated with analyte-sepcific antibody differntiated by APC fluorescence intensity (middle), and fluorescence signal in propotion to concentration of the analyte was measured for each cytokine in PE channel (right). Data was generated on Beckman Cytoflex S Flow Cytometer, equipped with blue laser (488 nm) and red laser (640 nm).
典型数据-Typical Data
Please refer to DS document for the assay protocol.
A double logarithmic fitting curve was plotted for each cytokine( IL-2, IL-4, IL-6, IL-10, TNF-α, IFN-γ). The present curve of each cytokines was ranging from 9.8 pg/ml to 10000 pg/ml respectively .
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