Human iPSC-Derived Intestinal Organoid Maintenance Kit

产品示意（Product Diagram）

Protocol Diagram of Intestinal organoid differentiation.

Organoid Histology and State

The Intestinal organoids differentiated using the Human iPSC-Derived Intestinal Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) show regular peristalsis.

Orgnaoid Histology

Observation of granulocyte like cells, adipocyte like cells, smooth muscle like cells and intestinal crypt like structure by morphology on day 120 Intestinal organoids.

Marker Expression

The Intestinal organoids differentiated using the Human iPSC-Derived Intestinal Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) show expression of enterochromaffin cells (CHGA) and endothelial cells (CD31).

The Intestinal organoids differentiated using the Human iPSC-Derived Intestinal Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) show expression of goblet cells (mucus-producing, MUC2) and endothelial cells (CD31).

The Intestinal organoids differentiated using the Human iPSC-Derived Intestinal Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) show expression of smooth muscle cell (α-SMA); epithelial cell (EPCAM); macrophage (CD68); goblet cell (MUC2); Ki67 (intestinal stem cell) and enteroendocrine cells (CHGA).

Organoid Activity

Intestinal organoids differentiated using the Human iPSC-Derived Intestinal Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) show normal intestinal function validated by the absorption of fatty acid and glucose.