疱疹病毒疫苗研发解决方案

The purity of Varicella zoster virus (strain Oka vaccine) gH&gL Protein, Twin-Strep Tag&His Tag (Cat. No. GHL-V5283) is more than 90% and the molecular weight of this protein is around 105-135 kDa verified by SEC-MALS.

The purity of Varicella zoster virus (strain Oka vaccine) gI≧ Protein, His Tag&Twin-Strep Tag (Cat. No. GIE-V5283) was greater than 85% as determined by SEC-HPLC.

The purity of HSV-2 (strain 333) Envelope Glycoprotein C (gC), His Tag (Cat. No. GLC-V52H3) is more than 90% and the molecular weight of this protein is around 45-60 kDa verified by SEC-MALS.

The purity of HSV-1 (strain 17) gH&gL Protein, Twin-Strep Tag&His Tag (Cat. No. GHL-H5283) is more than 85% and the molecular weight of this protein is around 130-160 kDa verified by SEC-MALS.

The purity of Epstein-Barr virus Glycoprotein H&Glycoprotein L Heterodimer Protein, Twin-Strep&His Tag (Cat. No. GHL-E5283) is more than 90% and the molecular weight of this protein is around 100-125kDa verified by SEC-MALS.

The purity of Epstein-Barr virus Envelope Glycoprotein B (gB), His Tag (Cat. No. GLB-E52H3) is more than 85% and the molecular weight of this protein is around 275-305 kDa verified by SEC-MALS.

The purity of HHV-5 (strain AD169) Glycoprotein H&L, His Tag (Cat. No. GHL-H5243) is more than 90% and the molecular weight of this protein is around 210-250 kDa verified by SEC-MALS.

The purity of HCMV (strain AD169) Glycoprotein B (gB), His Tag (Cat. No. CMB-V52H4) is more than 95% and the molecular weight of this protein is around 308-376 kDa verified by SEC-MALS.

Anti-Glycoprotein E (VZV) Antibody, Human IgG1 (1B10) (Cat. No. VZV-M544) captured on Protein A Chip can bind Varicella zoster virus (strain Oka vaccine) Envelope Glycoprotein E (gE), His Tag (Cat. No. GLE-V52H3) with an affinity constant of 5.63 nM as determined in SPR assay (Biacore 8K) (Routinely tested).

Immobilized HSV-2 (strain 333) Envelope Glycoprotein C (gC), His Tag (MALS verified) (Cat. No. GLC-V52H3) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-gC-HSV-2 (strain 333) Antibody, Mouse IgG1 (3A12) (Cat. No. HSV-Y184) with a linear range of 0.098-6.25 ng/mL (QC tested).

Detection of Monoclonal Glycoprotein E (VZV) titer by sandwich-ELISA Assay.
Immobilized Anti-Glycoprotein E (VZV) Antibody can bind Glycoprotein E (VZV). Detection was performed using Biotin-Anti-Glycoprotein E (VZV) Antibody with sensitivity of 78 pg/mL (QC tested).

For determination of antibody titer: the positive sample was diluted with a gradient, and the antibody titer of the sample corresponds to the highest dilution factor that still yields a positive reading.

The specific Anti-gC-HSV-2 (strain 333) Antibody pair (Clone:9A12/3A12) for the detection of HSV-2 (strain 333) Envelope Glycoprotein C (gC), His Tag (MALS verified) (Cat. No. GLC-V52H3), linear range of 1.56-0.024ng/mL. (sandwich ELISA)

For determination of antibody titer: the positive sample was diluted with a gradient, and the antibody titer of the sample corresponds to the highest dilution factor that still yields a positive reading.