AAV8 Titration ELISA Kit (Fast)

产品信息

产品描述（Product Details）

Assay Type	Sandwich-ELISA
Analyte	AAV8
Format	96T
Regulatory Status	RUO
Sensitivity	<1.94E+08capsids/mL
Standard Curve Range	6.22E+09 capsids/mL-1.94E+08 capsids/mL
Assay Time	2hr 20 min
Suitable Sample Type	For the detection and quantitative determination of AAV8 capsid titration in AAV gene therapy product preparation processing.
Sample volume	100 uL

组分（Materials Provided）

ID	Components	Size
AAV08H-C01	Pre-coated Anti-AAV8 Antibody Microplate	1 plate
AAV08H-C02	AAV8 Standard	1.4E+10 capsids
AAV08H-C03	HRP-Anti-AAV8 Antibody	15 μg
AAV08H-C04	10×Washing Buffer	50 mL
AAV08H-C05	2×Dilution Buffer	50 mL
AAV08H-C06	Substrate Solution	12 mL
AAV08H-C07	Stop Solution	7 mL

产品数据图

典型数据-Typical Data Please refer to DS document for the assay protocol.

For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.

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背景

The adeno-associated virus (AAV) is a small (25 nm), non-enveloped virus of the parvoviridae family, including 12 different AAV serotypes. In the parvoviridae family it belongs to the genus dependoparvovirus, because it needs the presence of a helper virus for replication and assembly. The icosahedral AAV capsid composed of the capsid proteins VP1, VP2 and VP3 contains a linear, single-stranded DNA genome of 4.7 kb.