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AAV8 Titration ELISA Kit (Fast)

COADSMS
DS中文
DS
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AAV-A008H-96tests
¥6825.00
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产品详情

  • 产品参数（Product Specifications）

    Assay Type
    Sandwich-ELISA
    Analyte
    AAV8
    Format
    96T
    Regulatory Status
    RUO
    Sensitivity
    <1.94E+08capsids/mL
    Standard Curve Range
    6.22E+09 capsids/mL-1.94E+08 capsids/mL
    Assay Time
    2hr 20 min
    Suitable Sample Type
    For the detection and quantitative determination of AAV8 capsid titration in AAV gene therapy product preparation processing.
    Sample volume
    100 uL

  • 产品概述（Product Overview）

    AAV8 Titration ELISA Kit (Fast) is based on the sandwich-ELISA method and is used to detect and quantitatively determine the AAV8 capsid titration in AAV gene therapy product winthin 2hr 20 min. The kit is designed to provide reliable solutions for the early development, production, and quality control of AAV gene therapy products.

  • 重构方法（Reconstitution）

    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

  • 存储（Storage）

    The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.

    The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • 组分（Materials Provided）

    ID
    Components
    Size
    AAV08H-C01
    Pre-coated Anti-AAV8 Antibody Microplate
    1 plate
    AAV08H-C02
    AAV8 Standard
    1.4E+10 capsids
    AAV08H-C03
    HRP-Anti-AAV8 Antibody
    15 μg
    AAV08H-C04
    10×Washing Buffer
    50 mL
    AAV08H-C05
    2×Dilution Buffer
    50 mL
    AAV08H-C06
    Substrate Solution
    12 mL
    AAV08H-C07
    Stop Solution
    7 mL

  • 质量管理控制体系（QMS）

    1. 质量管理体系（ISO, GMP）
    2. 质量优势
    3. 质控流程

数据展示

  • 典型数据-Typical Data

    Please refer to DS document for the assay protocol.

    AAV8 TYPICAL DATA

    For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.

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背景介绍

The adeno-associated virus (AAV) is a small (25 nm), non-enveloped virus of the parvoviridae family, including 12 different AAV serotypes. In the parvoviridae family it belongs to the genus dependoparvovirus, because it needs the presence of a helper virus for replication and assembly. The icosahedral AAV capsid composed of the capsid proteins VP1, VP2 and VP3 contains a linear, single-stranded DNA genome of 4.7 kb.

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