收藏产品 产品信息
优势特色（Features）
- Designed under ISO 9001:2015 and ISO 13485:2016
- Manufactured and QC tested under a GMP compliance factory
- Animal-Free materials
- Beta-lactam materials free
- Batch-to-batch consistency
- Stringent quality control tests
- No animal derived peptone and lactose used in production process
表达区间及表达系统（Source）
GMP Human BMP-4 (improved sequence) Protein (GMP-BM4H18) is expressed from E. coli cells. It is a variant of BMP-4 to suit cell culture applications better.
Predicted N-terminus: Met
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries no "tag".
The protein has a calculated MW of 13.2 kDa. The protein migrates as 13 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
内毒素（Endotoxin）
Less than 50 EU/mg by the LAL method.
宿主蛋白残留（Host Cell Protein）
<0.5 ng/µg of protein tested by ELISA.
宿主核酸残留（Host Cell DNA）
<0.02 ng/μg of protein tested by qPCR.
无菌（Sterility）
The sterility testing was performed by membrane filtration method described in CP<1101>, USP<71> and Eur. Ph. 2.6.1.
支原体（Mycoplasma）
Negative.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 20 mM Citric acid, pH2.2 with protectants.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with blue ice, please inquire the shipping cost.
存储（Storage）
Upon receipt, store it immediately at -20°C or lower for long term storage.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 5 years in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
产品数据图
电泳（SDS-PAGE）
GMP Human BMP-4 (improved sequence) Protein on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-CELL BASE
GMP Human BMP-4 (improved sequence) Protein (Cat. No. GMP-BM4H18) stimulates Human BMP (Luc) HEK293 Reporter Cell. The specific activity of GMP Human BMP-4 (improved sequence) Protein is > 1.90 x 10^3 U/mg (QC tested).
Protocol
应用数据（Application Data）
GMP Human BMP-4 (improved sequence) Protein (Cat. No. GMP-BM4H18) could significantly induce iPSC derived HSPC differentiation, with high expression of CD34 and CD45 (HSPC markers) by flow cytometry analysis and the increase of viable numbers of CD34+ CD45+ cells by cell counting. GMP Human BMP-4 (improved sequence) Protein (Cat. No. GMP-BM4H18) has a similar performance with Human BMP-4 (improved sequence) Protein, premium grade (Cat. No. BM4-H5118).
稳定性（Stability）
The Cell based assay shows that GMP Human BMP-4 (improved sequence) Protein (Cat. No. GMP-BM4H18) is stable at 37°C for 7 hours.
The Cell based assay shows that GMP Human BMP-4 (improved sequence) Protein (Cat. No. GMP-BM4H18) is stable after freezing and thawing 3 times.
The Cell based assay shows batch-to-batch consistency between Acro's GMP and PG BMP-4 (improved sequence).
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背景
Bone Morphogenetic Protein 4 (BMP4) is a member of growth factor of the TGF-beta superfamily that plays essential roles in many developmental processes, including neurogenesis, vascular development, angiogenesis and osteogenesis. BMP-4 Initiates the canonical BMP signaling cascade by associating with type I receptor BMPR1A and type II receptor BMPR2. BMP-4 can acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction.
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