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过表达细胞系

基因过表达是指通过慢病毒、电转等细胞转染方式，提高某特定基因的表达水平，从而实现基因的功能增强（gain of function）。基因过表达作为一种传统的分子生物学技术，被认为是研究基因功能的实验策略之一，至今依然在生物学研究中发挥着重要作用。过表达稳定细胞系（Overexpression Stable Cell Lines）则是基于某一细胞系构建的用于实现某特定基因的持续过表达的产物。过表达细胞系通过将目的基因构建到表达载体上，通过慢病毒侵染等方式导入宿主细胞，整合到染色体上，使目的抗原在宿主细胞膜表面进行长期稳定的表达。
ACROBiosystems百普赛斯基于成熟的细胞构建平台，持续开发高质量的过表达细胞系产品，为新药研发提供一个稳定的细胞水平研发工具！

应用场景

  1. >基因功能研究
  2. >免疫/杀伤/增殖等
  3. >抗体药物的活性筛选（细胞水平的结合和阻断）
  4. >CAR分子的杀伤活性评价

产品列表

验证数据

Receptor Assay
CAR-T靶细胞开发

Expression analysis of human CD64 on CHO/Human CD64 Stable Cell Line by FACS. Cell surface staining using PE-labeled anti-human CD64 antibody was performed on CHO/Human CD64 Stable Cell Line with different expression levels: CHO/Human CD64 Stable Cell Line (Low Expression) (Cat. No. SCCHO-ATP062L); CHO/Human CD64 Stable Cell Line (Medium Expression) (Cat. No. SCCHO-ATP062M); CHO/Human CD64 Stable Cell Line (High Expression) (Cat. No. SCCHO-ATP062H).

Application
单抗活性筛选

Bioactivity analysis of anti-human 4-1BB antibody through CHO/Human CD32b Stable Cell Line (Medium Expression) (Cat. No. SCCHO-ATP060M) crosslinking to test whether in a CD32b-dependent manner to strengthen the agonistic activity. The EC50 of anti-human 4-1BB antibody is approximately 0.18 μg/mL through CHO/Human CD32b Stable Cell Line (Medium Expression) crosslinking.

单抗活性筛选

Blocking activity of anti-human PD-1 antibody (FOLD). This Raji/Human PD-L1 Stable Cell Line (Cat. No. SCRAJ-STT075) was incubated with serial dilutions of antibodies in the presence of reporter cells expressing human PD-1. The max induction fold was approximately 22.47.

  • 背景介绍
  • 产品列表
  • 验证数据