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组分（Materials Provided）
Box A(2-8°C)ID Components Size RIPO-HWM002K-C01 Basal medium A 5.4ml RIPO-HWM002K-C02 Basal medium B 22.5ml RIPO-HWM002K-C03 Basal medium C 27ml RIPO-HWM002K-C04 Basal medium M-M 22.5ml 产品描述（Product Details）
Human iPSC-Derived Cardiac Organoid differentiation Kit (Catalog No#: RIPO-HWM002K) allows hESC or iPSC to differentiate into cardiac organoids. Cardiac organoids are three-dimensional in vitro models with a cellular composition and structural organization that is representative of the human heart. This kit can produce 24 cardiac organoids in four steps. Differentiation was carried out by forming EBs from PSC at an ultra-low adsorption interface, and then changing the medium according to the instructions. A spontaneously beating cardiac organoid with a cavity can be seen between days 9 and 13 of the onset of differentiation. Cardiomyocyte, key cellular component of cardiac organoid, are responsible for the beating of the cardiac organoids. The beating rate of the formed cardiac organoids increases dramatically when stimulated with isoproterenol or forskolin, and there is a linear relationship between the concentration of the drug and the beating rate over a range of concentrations.
产品特征（Product Specification）
The basic medium of this differentiation kit is a serum-free, well-defined medium with minimal batch variation to which differentiation factors are added. This medium does not contain antibiotics, the addition of which may affect cardiac organoid differentiation.
存储（Storage）
The unopened Box A is stable for 12 months from the date of manufacture if stored under 2-8°C.
The unopened Box B is stable for 12 months from the date of manufacture if stored under -20°C.
The opened kit should be stored according to components table.- *请注意，为了支持完整的类器官培养和维持过程，本产品必须与Human iPSC-Derived Cardiac Organoid Maintenance Kit (Cat. No. RIPO-HWM004）一起使用。您可以点击此链接获取产品信息。
质量管理控制体系（QMS）
Box B(-20°C)
| ID | Components | Size |
| RIPO-HWM002K-1-C01 | Medium F | 3ml |
| RIPO-HWM002K-1-C02 | Supplement A | 0.6ml |
| RIPO-HWM002K-1-C03 | Supplement B | 2.5ml |
| RIPO-HWM002K-1-C04 | Supplement C | 3ml |
| RIPO-HWM002K-1-C05 | Supplement M-M | 2.5ml |
产品数据图
产品示意（Product Diagram）
Protocol Diagram of cardiac organoid differentiation.
The cardiac organoids differentiated using the Human iPSC-Derived Cardiac Organoid Differentiation Kit (Ca. No. RIPO-HWM002K) show regular beating from day 7-13 of differentiation.
The cardiac organoids differentiated using the Human iPSC-Derived Cardiac Organoid Differentiation Kit (Ca. No. RIPO-HWM002K) demonstrate the expression of cardiomyocyte marker cTnT (green).
The electrical activity of the cardiac organoids differentiated using the Human iPSC-Derived Cardiac Organoid Differentiation Kit (Ca. No. RIPO-HWM002K) was recorded using silicon probes (Ca. No. P64-7), revealing electrophysiological activity that closely resembles that of the actual human heart.
Organoid Histology and State
Marker Expression
Organoid Activity
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