Human iPSC-Derived Intestinal Organoid Differentiation Kit

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RIPO-IWM005K-1kit
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产品信息

  • 组分(Materials Provided)

    Box A(2-8°C)
    ID
    Components
    Size
    RIPO-IWM005K-C01
    Intestinal organoid Basal Medium B
    14.5ml
    RIPO-IWM005K-C02
    Intestinal organoid Basal Meidium C
    14.5ml
    RIPO-IWM005K-C03
    Intestinal organoid Basal Medium D
    90ml
    Box A(2-8°C)
    ID
    Components
    Size
    RIPO-IWM005K-1-C01
    Intestinal organoid Medium A
    10ml
    RIPO-IWM005K-1-C02
    Intestinal organoid Supplement B
    1.5ml
    RIPO-IWM005K-1-C03
    Intestinal organoid Supplement C
    1.5ml
    RIPO-IWM005K-1-C04
    Intestinal organoid Supplement D
    10ml
  • 产品描述(Product Details)

    Human iPSC-Derived Intestine Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) allows hESC or hiPSC to differentiate into intestine organoids. Intestine organoids are three-dimensional in vitro models with a cellular composition and structural organization that is representative to the human intestine regions. This kit can produce 48 intestinal organoids in four steps. Organoids generated using Human iPSC-Derived intestine Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) feature various types of cells, including intestine epithelium cells, mesenchyme cells, enterocytes, Paneth cells, goblet cells, etc. These intestine organoids show intestine crypt like structure, Villi and Microvilli like structure. These intestine organoids show normal intestinal function validated by the absorption of fatty acid and glucose.

  • 产品特征(Product Specification)

    The basic medium of this differentiation kit is a serum-free, well-defined medium with minimal batch variation to which differentiation factors are added. This medium does not contain antibiotics, the addition of which may affect organoid differentiation.

  • 存储(Storage)

    The unopened Box A is stable for 12 months from the date of manufacture if stored under 2-8°C.
    The unopened Box B is stable for 12 months from the date of manufacture if stored under -20°C.
    The opened kit should be stored according to components table.

  • 质量管理控制体系(QMS)

    质量管理体系(ISO, GMP)

    质量优势

    质控流程

*请注意,为了支持完整的类器官培养和维持过程,本产品必须与Human iPSC-Derived Intestinal Organoid Maintenance Kit (Cat. No. RIPO-IWM006)一起使用。您可以点击此链接获取产品信息。

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  • 产品示意(Product Diagram)

    1

    Protocol Diagram of intestine organoid differentiation.

验证数据(Validation Data)

  • Organoid Histology and State

    The intestine organoids differentiated using the Human iPSC-Derived intestine Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) show regular peristalsis.

  • Orgnaoid Histology

    Observation of granulocyte like cells, adipocyte like cells, smooth muscle like cells and intestinal crypt like structure by morphology on day 120 intestine organoids.

  • Marker Expression

    The intestine organoids differentiated using the Human iPSC-Derived intestine Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) show expression of enterochromaffin cells (CHGA) and endothelial cells (CD31).

    The intestine organoids differentiated using the Human iPSC-Derived intestine Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) show expression of goblet cells (mucus-producing, MUC2) and endothelial cells (CD31).

    The intestine organoids differentiated using the Human iPSC-Derived intestine Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) show expression of smooth muscle cell (α-SMA); epithelial cell (EPCAM); macrophage (CD68); goblet cell (MUC2); Ki67 (intestinal stem cell) and enteroendocrine cells (CHGA).

  • Organoid Activity

    Intestine organoids differentiated using the Human iPSC-Derived intestine Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) show normal intestinal function validated by the absorption of fatty acid and glucose.

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