GMP GENIUS™NucleaseDMF

优势特色（Features）

1. Designed under ISO 9001:2015 and ISO 13485:2016
2. Manufactured and QC tested under a GMP compliance factory
3. FDA DMF filed
4. Animal-Free materials
5. Beta-lactam materials free
6. Batch-to-batch consistency
7. Stringent quality control tests
8. No animal derived peptone and lactose used in production process

产品描述（Product Details）

GMP GENIUS™Nuclease is a tag-free recombinant form of Serratia marcescens extracellular endonuclease produced in Escherichia coli using a proprietary process at ACRObiosystems. GMP GENIUS™Nuclease performs optimally at low and physiological salt concentrations (0-200 mM NaCl). The product is a homodimer with monomer molecular masses of about 30 kDa. The enzyme is a non-specific nuclease with high specific activity, degrading single- and double-stranded nucleic acids in any form (single stranded, double stranded, linear, circular, and supercoiled). It hydrolyzes internal phosphodiester bonds present between the nucleotides to 5’- phosphorylated oligonucleotides of 3-5 bases in length.

应用说明（Application）

Elimination of nucleic acids from biologics, optimal for samples with 0-200 mM salt in presence of 2-10 mM Mg++

• clinical viral vaccine production

• clinical viral vector production for cell and gene therapy (CGT)

• Other clinical development and production uses

反应条件（Operating Conditions）

GMP GENIUS™Nuclease is functional between pH 6 and 10 (optimal at pH8 - 8.5) , and from 0°C to 50 °C (optimal at 37 °C - 45 °C). Mg2+ (1-2 mM) is required for enzyme activity.1 mM EDTA reduced the activity by 30% in the presence of 1 mM MgCl2; 0.1 M EDTA eliminated all enzyme activity. In the presence of 1 mM MgCl2, enzyme levels were reduced 75% by 0.1 M CaCl2 or 1 M NaCl. Under standard assay conditions, 1 mM iodoacetate had no effect on the enzymatic rate, whereas 1 mM mercaptoethanol and maleic acid reduced the activity by only 5 to 10%. 10 mM p- Chloromercurybenzoate completely inactivates the enzyme, while 0.64 M beta-mercaptoethanol in the presence of 2 M urea causes only partial inactivation of the enzyme. 4 or 7 M Urea increases the enzyme activity.

纯度（Purity）

>95% as determined by SDS-PAGE.

>99% as determined by SEC-HPLC.

酶活（Enzyme Activity）

≥250 U/μL

宿主蛋白残留（Host Cell Protein）

<0.05 ng/µg of protein tested by ELISA.

蛋白酶活性（Protease Activity）

Negative.

无菌（Sterility）

The sterility testing was performed by membrane filtration method described in CP<1101>, USP<71> and Eur. Ph. 2.6.1.

支原体（Mycoplasma）

Negative.

内毒素（Endotoxin）

Less than 10 EU/mg by the LAL method.

重金属残留（Heavy Metals）

≤10 ppm

制剂（Formulation）

Supplied as 0.2 μm filtered solution in 20 mM Tris, 20 mM NaCl, 2 mM MgCl2, pH8.0.

Contact us for customized product form or formulation.

运输（Shipping）

This product is supplied and shipped with dry ice, please inquire the shipping cost.

存储（Storage）

This product is stable after storage at:

1. The product MUST be stored at -20°C or lower upon receipt;
2. -20°C for 5 years under sterile conditions.