resDetect™ Kanamycin ELISA Kit

产品描述（Product Details）

背景（Background）

Residues of kanamycin are prone to occur in the production process of biological products, which can easily lead to abnormal reactions in the human body. Therefore, the residual amount of kanamycin in biological products or semi-finished products of biological products should be strictly controlled.

应用说明（Application）

The Kanamycin ELISA Kit is calibrated against the NIFDC standard.

The kit is developed for the detection and quantitative determination of kanamycin residues in plasmid DNA raw materials and proteins for CGT, vaccine and other biological drugs.

It is for research use only.

重构方法（Reconstitution）

Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

存储（Storage）

1. The kit should be stored at 2°C to 8°C upon receiving.

2. The reconstructed kanamycin standard is stored at -70°C in at least 300 uL per tube and cannot be frozen and thawed repeatedly.

3. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

组分（Materials Provided）

原理（Assay Principles）

The Kanamycin quantitative detection kit adopts the indirect competitive ELISA method, and the pre-coated conjugated kanamycin antigen on the microstrip competes with the residual kanamycin in the sample to bind the enzyme-labeled anti-kanamycin monoclonal antibody, and then uses a microplate reader to detect the absorbance value by adding TMB substrate, and the absorbance value is negatively correlated with the content of kanamycin in the sample. The kit takes only about one hour and 20 minutes to operate and has a linear range of 0.5 ng/mL to 40.5 ng/mL.