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产品信息
优势特色（Features）
- Designed under ISO 9001:2015 and ISO 13485:2016
- Manufactured and QC tested under a GMP compliance factory
- FDA DMF filed
- Animal-Free materials
- Beta-lactam materials free
- Batch-to-batch consistency
- Stringent quality control tests
表达区间及表达系统（Source）
GMP Human IL-2 Protein (GMP-L02H14) is expressed from E. coli cells. It contains AA Ala 21 - Thr 153 (Accession # P60568-1).
Predicted N-terminus: Met
蛋白结构（Molecular Characterization）
This protein carries no "tag".
The protein has a calculated MW of 15.4 kDa. The protein migrates as 16 kDa±2 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
内毒素（Endotoxin）
Less than 5.0 EU/ Vial by the LAL method.
宿主蛋白残留（Host Cell Protein）
<0.5 ng/µg of protein tested by ELISA.
宿主核酸残留（Host Cell DNA）
<0.1 ng/μg of protein tested by qPCR.
无菌（Sterility）
The sterility testing was performed by membrane filtration method described in CP<1101>, USP<71> and Eur. Ph. 2.6.1.
支原体（Mycoplasma）
Negative.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in phosphate with protectants.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with blue ice, please inquire the shipping cost.
存储（Storage）
Upon receipt, store it immediately at -20°C or lower for long term storage.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C for 5 years in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
产品数据图
电泳（SDS-PAGE）
GMP Human IL-2 Protein on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-Bioactivity CELL BASE
GMP Human IL-2 Protein (Cat. No. GMP-L02H14) stimulates proliferation of CTLL-2 cells. The specific activity of GMP Human IL-2 Protein is ≥ 1.20×10^7 IU/mg, which is calibrated against human Interleukin-2 China National Standard (NIFDC code: 270008) (QC tested). China National Institutes for Food and Drug Control (NIFDC) Standard was prepared and calibrated against human IL-2 WHO International Standard (NIBSC code: 86/500) by NIFDC.
Protocol
应用数据（Application Data）
3 × 10^6 Human PBMCs were cultured with various concentrations of GMP Human IL-2 Protein (ACROBiosystems, Cat. No. GMP-L02H14) or IL-2 Protein (Competitor N) for a week. For the negative control group (w/o IL-2), the CD3+ ratio was 47.6%, and the CD3+ cell number was 4.8 × 10^5. The result shows that GMP Human IL-2 Protein (ACROBiosystems, Cat. No. GMP-L02H14) has a similar activity to IL-2 Protein (Competitor N).
3 × 10^6 Human PBMCs were cultured with various concentrations of GMP Human IL-2 Protein (ACROBiosystems, Cat. No. GMP-L02H14) or IL-2 Protein (Competitor N) for a week. For negative control group (w/o IL-2), the CD3+ CD4- CD8+ ratio was 21.7%, and the CD3+ CD4- CD8+ cell number was 1.0 × 10^5. The result shows that GMP Human IL-2 Protein (ACROBiosystems, Cat. No. GMP-L02H14) has a similar activity to IL-2 Protein (Competitor N).
3 × 10^6 Human PBMCs were cultured with various concentrations of GMP Human IL-2 Protein (ACROBiosystems, Cat. No. GMP-L02H14) or IL-2 Protein (Competitor N) for a week. For negative control group (w/o IL-2), the CD3+ CD4+ CD8- ratio was 72.4%, and the CD3+ CD4+ CD8- cell number was 3.5 × 10^5. The result shows that GMP Human IL-2 Protein (ACROBiosystems, Cat. No. GMP-L02H14) has a similar activity to IL-2 Protein (Competitor N).
稳定性（Stability）
The Cell based assay shows that GMP Human IL-2 Protein (Cat. No. GMP-L02H14) is stable at 37°C for 8 hours.
The Cell based assay shows that GMP Human IL-2 Protein (Cat. No. GMP-L02H14) is stable in the quality of different batches.
The Cell based assay shows that GMP Human IL-2 (Cat. No. GMP-L02H14) is stable after freezing and thawing 3 times.
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背景
Interleukin-2 (IL-2) is an interleukin, a type of cytokine immune system signaling molecule, which is a leukocytotrophic hormone that is instrumental in the body's natural response to microbial infection and in discriminating between foreign (non-self) and self. IL-2 mediates its effects by binding to IL-2 receptors, which are expressed by lymphocytes, the cells that are responsible for immunity. Mature human IL-2 shares 56% and 66% aa sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit crossspecies activity. The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes. IL-2 is also necessary during T cell development in the thymus for the maturation of a unique subset of T cells that are termed regulatory T cells (T-regs). After exiting from the thymus, T-Regs function to prevent other T cells from recognizing and reacting against "self antigens", which could result in "autoimmunity". T-Regs do so by preventing the responding cells from producing IL-2. Thus, IL-2 is required to discriminate between self and non-self, another one of the unique characteristics of the immune system.
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