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resDetect™ HIV-1 p24 ELISA Kit (Residue Testing)

COADSMS
DS中文
DS
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RES-A050-96tests
¥2940.00
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产品详情

  • 产品参数（Product Specifications）

    Assay Type
    Sandwich-ELISA
    Analyte
    Capsid protein p24
    Format
    96T(8×12 strips)
    Reactivity
    HIV (HV1-HXB2)
    Regulatory Status
    RUO
    Sensitivity
    <7.81pg/mL
    Standard Curve Range
    250-7.81pg/mL
    Assay Time
    3hr 20 min
    Suitable Sample Type
    For the quantitative determination of HIV-1 p24 in Cell Culture Supernatants, Plasma, Serum.
    Sample volume
    100 uL

  • 产品概述（Product Overview）

    resDetect™ HIV-1 p24 ELISA Kit (Residue Testing) is based on the ELISA sandwich method and is used for the quantitative detection of Human Immunodeficiency Virus-1 p24 (HIV-1 p24) in samples from CAR-T product preparation processes where it is used as a lentivirus vector. It is designed to provide a reliable solution for CAR-T product quality assessment during drug development and CMC quality control stages.

  • 重构方法（Reconstitution）

    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

  • 存储（Storage）

    1. Unopened kit should be stored at 2℃-8℃ upon receiving.

    2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

    3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • 组分（Materials Provided）

    ID
    Components
    Size
    RES050-C01
    Pre-coated Anti-HIV-1 p24 Antibody Microplate
    1 plate(8×12 strips)
    RES050-C02
    HIV-1 p24 Standard
    20 μg
    RES050-C03
    Biotin-Anti-HIV-1 p24 Antibody
    20 μg
    RES050-C04
    Streptavidin-HRP
    50 μL
    RES050-C05
    10xWashing Buffer
    50 mL
    RES050-C06
    2xDilution Buffer
    50 mL
    RES050-C07
    Substrate Solution
    12 mL
    RES050-C08
    Stop Solution
    7 mL
    RES050-C09
    1xLysis Buffer
    12 mL

  • 质量管理控制体系（QMS）

    1. 质量管理体系（ISO, GMP）
    2. 质量优势
    3. 质控流程

数据展示

  • 典型数据-Typical Data

    Please refer to DS document for the assay protocol.

    Capsid protein p24 TYPICAL DATA

    For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.

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背景介绍

Human Immunodeficiency Virus (HIV) can be divided into two major types, HIV type 1 (HIV-1) and HIV type 2 (HIV-2). HIV-1 is related to viruses found in chimpanzees and gorillas living in western Africa. The gag gene of HIV-1 encodes a precursor protein known as Pr55Gag. The viral protease PR cleaves this precursor to generate p17, p24, p7, and p6 proteins which are required for virus particle assembly. p24 is a major viral core structural protein, which is the main component of HIV virus and plays an important role in the process of virus reproduction. Usually, p24 protein can be detected in blood 2-3 weeks after hiv infection.Its measurement is commonly used as an indicator of HIV-1 infection and viral load. Therefore, it's helpful to develop the Human Immunodeficiency Virus type 1 (HIV-1) p24 ELISA Kit to quantitative detection the HIV-1 p24 antigen in cell culture supernates .

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