Streptavidin(SA)系列产品

1963年,Stapley首次从阿维丁链霉菌(Streptomyces avidinii)培养液中分离得到链霉亲和素 (Streptavidin,SA)。作为已知自然界中最强的非共价相互作用之一,SA同源四聚体与生物素(Biotin)可以以高亲合力牢固结合并产生多级放大效应,这使得SA- Biotin系统成为生物化学实验中被广泛应用的宝贵资产。
SA-Biotin系统常被偶联到抗体或蛋白,其中SA偶联标记物如HRP、荧光基团等,Biotin被偶联到抗体或蛋白(MABSOL®生物素标记蛋白),通过SA-Biotin桥连来实现间接检测靶标,广泛用于微量抗原和抗体定性、定量检测及定位观察研究的新技术,使得免疫标记和有关示踪分析更加灵敏。
ACROBiosystems可提供SA系列产品:重组SA蛋白,标记SA蛋白,SA包被板,SA磁珠等,助力您的科研项目和药物研发。其中,标记SA蛋白包含有PG级别的PE labeled SA产品,严格无菌,可以用于细胞等相关实验的临床前和临床应用。

SA系列产品

SA系列产品验证数据

PE标记SA蛋白验证数据(FACS)

PE标记SA蛋白验证数据(FACS)

5e5 of Anti-CD19 CAR-293 cells were stained with 100μL of 20 μg/mL Biotinylated Human CD19 (20-291), Fc, Avitag, premium grade (Cat. No. CD9-H82F6) and negative control protein respectively, washed and then followed with 2.5 μg/mL of Streptavidin Protein-PE, premium grade (Cat. No. STN-NP117) and analyzed with FACS. PE signal was used to evaluate the binding activity (QC tested).

Protocol

PE标记SA蛋白验证数据(FACS)

5e5 of Anti-CD19 CAR-293 cells were stained with 100μL of 20 μg/mL Biotinylated Human CD19 (20-291) Protein, Fc, Avitag, premium grade (Cat. No. CD9-H82F6) and negative control protein respectively, washed and then followed with 2.5 μg/mL of Streptavidin Protein-PE, premium grade (Cat. No. STN-NP117) and analyzed with FACS. PE signal was used to evaluate the binding activity (QC tested).

Protocol

SA蛋白活性(ELISA)

STN-N5116-E1

Immobilized Biotinylated Human CD19 (20-291), His,Avitag (Cat. No. CD9-H82E9) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Monoclonal Anti-Human CD19 Antibody, Mouse IgG2a with a linear range of 0.1-6 ng/mL (QC tested).

Protocol

STN-N5116-E2

Immobilized Biotinylated SARS-CoV-2 S protein RBD, His,Avitag (Cat. No. SPD-C82E9) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Monoclonal Anti-SARS-CoV-S protein RBD Antibody, Human IgG1 with a linear range of 0.1-4 ng/mL (Routinely tested).

Protocol

SA-HRP应用数据

SA-HRP应用数据

Immobilized Cynomolgus / Rhesus macaque BCMA, His Tag (Cat. No. BCA-C52H7) at 1 μg/mL (100 μL/well) can bind Biotinylated Human APRIL, Fc,Avitag (Cat. No. APL-H82F5) with a linear range of 0.5-16 ng/mL when detected by Streptavidin Protein-HRP, Horseradish peroxidase conjugated Streptavidin (0.1 µg/mL) (Cat. No. STN-NH913) (QC tested).

Protocol

SA-HRP应用数据

Measure performance comparison between Acro (Streptavidin Protein-HRP, Horseradish peroxidase conjugated Streptavidin) and other competitive products. With tight quality control measures, Acro has better sensitivity than other brands.

Protocol

SA 包被板应用数据

SP-11-E1

Immobilized Biotinylated Human CD19 (20-291), His,Avitag (Cat. No. CD9-H82E9) at 1 μg/mL (100 μL/well) on Streptavidin Coated Plates, Clear, 96-Well (Cat. No. SP-11), can bind Anti-FMC63 antibody with a linear range of 0.1-3 ng/mL (QC tested).

Protocol

SP-11-E2

Immobilized Biotinylated Human PD-1, Fc,Avitag,His Tag (Cat. No. PD1-H82F4) at 1 μg/mL (100 μL/well) on Streptavidin Coated Plates, Clear, 96-Well (Cat. No. SP-11), can bind Human PD-L1, mFc Tag with a linear range of 0.4-6.25 ng/mL (QC tested).

Protocol

SP-11-E3

Immobilized Biotinylated Human CD20 Full Length, His,Avitag (HEK293) (Cat. No. CD0-H82E5) at 1 μg/mL (100 μL/well) on Streptavidin Coated Plates, Clear, 96-Well (Cat. No. SP-11), can bind Rituximab with a linear range of 78-625 ng/mL (QC tested).

Protocol

SP-11-E4

Immobilized Biotinylated Human FCGRT&B2M Heterodimer Protein, His,Avitag (Cat. No. FCM-H82W7) at 1 μg/mL (100 μL/well) on Streptavidin Coated Plates, Clear, 96-Well (Cat. No. SP-11), can bind Ipilimumab with a linear range of 78-1250 ng/mL (QC tested).

Protocol

SA 磁珠应用数据

SMB-B01-E1

Immobilized 40 μg SARS-CoV-2 S1 protein to 1mg Beads (Cat. No. SMB-B01) can bind the Anti-SARS-CoV-S protein RBD Antibody with an EC50 1.157 μg/mL (QC tested).

Protocol

参考文献

  • Louis Chaiet, Frank J. Wolf. The properties of streptavidin, a biotin-binding protein produced by Streptomycetes, Archives of Biochemistry and Biophysics (1964). https://doi.org/10.1016/0003-9861(64)90150-X.

相关产品

  • SA-Biotin系统
  • SA系列产品
  • SA系列产品验证数据
  • 参考文献
  • 相关产品