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产品信息
优势特色（Features）
- Designed under ISO 9001:2015 and ISO 13485:2016
- Manufactured and QC tested under a GMP compliance factory
- Animal-Free materials
- Beta-lactam materials free
- Batch-to-batch consistency
- Stringent quality control tests
- No animal derived peptone and lactose used in production process
表达区间及表达系统（Source）
GMP Human IL-18 Protein (GMP-L18H16) is expressed from E. coli cells. It contains AA Tyr 37 - Asp 193 (Accession # Q14116).
Predicted N-terminus: Tyr 37
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries no "tag".
The protein has a calculated MW of 18.2 kDa. The protein migrates as 17 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
内毒素（Endotoxin）
Less than 10 EU/mg by the LAL method.
宿主蛋白残留（Host Cell Protein）
<0.5 ng/µg of protein tested by ELISA.
宿主核酸残留（Host Cell DNA）
<0.02 ng/μg of protein tested by qPCR.
无菌（Sterility）
The sterility testing was performed by membrane filtration method described in CP<1101>, USP<71> and Eur. Ph. 2.6.1.
支原体（Mycoplasma）
Negative.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with protectants.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with blue ice, please inquire the shipping cost.
存储（Storage）
Upon receipt, store it immediately at -20°C or lower for long term storage.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 5 years in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
产品数据图
电泳（SDS-PAGE）
GMP Human IL-18 Protein on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-Bioactivity CELL BASE
GMP Human IL-18 Protein (Cat. No. GMP-L18H16) stimulates secretion of IFN-γ by KG-1 cells. The specific activity of GMP Human IL-18 Protein is > 3.00ⅹ10^6 IU/mg, which is calibrated against WHO Reference Reagent Interleukin-18 (Human rDNA derived) (NIBSC code: 03/200) (QC tested).
Protocol
The activity of GMP Human IL-18 Protein (Cat. No. GMP-L18H16) was higher than other competing products.
应用数据（Application Data）
Human PBMCs were cultured with GMP Human IL-2 Protein (ACROBiosystems, Cat. No. GMP-L02H14), GMP Human IL-18 Protein (ACROBiosystems, Cat. No. GMP-L18H16) and GMP Human 4-1BB Ligand Protein (ACROBiosystems, Cat. No. GMP-41LH26), in CelThreaTM GMP T Cell Expansion Medium (ACROBiosystems, Cat. No. GMP-CM3101 & GMP-CM3101-1) for two weeks. The result shows that GMP Human IL-18 Protein can promote the expansion of these cells with a reasonable cell viability. Notably, the cells exhibit better expansion in GMP Human IL-18 Protein (ACROBiosystems).
Human PBMCs were cultured with GMP Human IL-2 Protein (ACROBiosystems, Cat. No. GMP-L02H14), GMP Human IL-18 Protein (ACROBiosystems, Cat. No. GMP-L18H16) and GMP Human 4-1BB Ligand Protein (ACROBiosystems, Cat. No. GMP-41LH26), in CelThreaTM GMP T Cell Expansion Medium (ACROBiosystems, Cat. No. GMP-CM3101 & GMP-CM3101-1) for two weeks. The result shows that GMP Human IL-18 Protein (ACROBiosystems) can be comparable with the Competitor M IL-18 protein.
稳定性（Stability）
The Cell based assay shows batch-to-batch consistency between Acro's GMP and PG IL-18.
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背景
Interleukin-18 (IL-18) is a potent proinflammatory cytokine that induces interferon-gamma (IFN-gamma) production from Th1 cells, NK cells and activated macrophages, particularly in the presence of IL-12. IL-18 also functions in developmental regulation of T-lymphocyte helper type I cells and in Fas-mediated cytotoxicity. Suppression of IL-18 activity is being investigated for treatment of chronic inflammatory diseases such as Crohn's disease and rheumatoid arthritis. It acts by inducing heterodimerization of the two subunits of its receptor, IL-18RAlpha and IL-18RBeta shows structural similarity to IL-1.
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