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VEGF蛋白：切断肿瘤的供养通道

血管内皮生长因子(Vascular endothelial growth factor, VEGF) 除了具有促进血管新生和再生等重要作用外，还具有免疫调节、神经保护、组织修复等多种功能。VEGF主要与受体VEGFR相互结合发挥功能。研究表明VEGF参与许多疾病的发生，在多种恶性肿瘤中均有表达，特别在肿瘤组织血管增生丰富的部位，因此成为药物开发的主要靶点。针对VEGF有100多种抗体药处于各种研发阶段，其中多款单抗药物已经上市，针对这一靶点的双抗药也在积极开发中。覆盖包括肿瘤、视觉性疾病以及心血管疾病等多种类型，具有极大潜力。
ACROBiosystems开发了多种VEGF家族蛋白产品。覆盖不同种属、不同亚型、不同标签或者无标签的蛋白产品，满足不同的实验需求。

产品列表

VEGF-D
VEGF-C
VEGF-B
VEGF-A
VEGF R3
VEGF R2
VEGF R1

验证数据

高纯度经MALS验证
The purity of ActiveMax® Human VEGF165, Tag Free (MALS verified) (Cat. No. VE5-H4210) is more than 95% in HP-SEC, and the molecular weight of this protein is around 40-55 kDa verified by SEC-MALS.
生物活性经ELISA&SPR&BLI&细胞实验等验证
>>ELISA

Immobilized Human VEGF R1&VEGF R2, Fc Tag at 2 μg/mL (100 μL/well) can bind Human VEGF165, His Tag (Cat. No. VE5-H5248) with a linear range of 0.004-0.063 μg/mL (QC tested).

Protocol

Immobilized Human VEGF165, premium grade (MALS verified) (Cat. No. VE5-H4210) at 0.1 μg/mL (100 μL/well) can bind Human VEGF R1 Protein, His Tag (Cat. No. VE1-H52H9) with a linear range of 1.2-10 ng/mL (QC tested).

Protocol

>>SPR

Immobilized Biotinylated Human VEGF165, His,Avitag (Cat. No. VE5-H82Q0) on CM5 Chip via Streptavidin, can bind Avastin with an affinity constant of 0.417 nM as determined in SPR assay (Biacore T200) (Routinely tested).

Protocol

>>BLI

Loaded Avastin (Bevacizumab) on AHC Biosensor, can bind Human VEGF165, His Tag (Cat. No. VE5-H5248) with an affinity constant of 0.448 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

>>细胞实验

ActiveMax® Human VEGF165, Tag Free (MALS verified) (Cat. No. VE5-H4210) stimulates proliferation of human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is 4.216-9.281 ng/mL (Routinely tested).

Protocol

Inhibition assay shows that the proliferation effect of ActiveMax® Human VEGF165, Tag Free (MALS verified) (Cat. No. VE5-H4210) is inhibited by increasing concentration of anti-VEGF mAb (Avastin). The concentration of VEGF165 used is 20 ng/mL. The ED50 is 0.065-0.229 μg/mL (Routinely tested).

Protocol

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