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优势特色（Features）
- Designed under ISO 9001:2015 and ISO 13485:2016
- Manufactured and QC tested under a GMP compliance factory
- FDA DMF filed
- Animal-Free materials
- Batch-to-batch consistency
- Stringent quality control tests
抗体来源（Source）
HEK293 cells-expressed human GMP Monoclonal Anti-Human CD3 Antibody (OKT3).
亚型（Isotype）
Mouse IgG2a/kappa
偶联（Conjugate）
Unconjugated
特异性（Specificity）
This product is a specific antibody specifically reacts with CD3 epsilon.
内毒素（Endotoxin）
Less than 2 EU/mg by the LAL method.
蛋白A残留（Protein A）
<5 ppm of protein tested by ELISA.
宿主蛋白残留（Host Cell Protein）
<0.5 ng/μg of protein tested by ELISA.
宿主核酸残留（Host Cell DNA）
<0.02 ng/μg of protein tested by qPCR.
纯度（Purity）
>95% as determined by SDS-PAGE.
>95% as determined by SEC-HPLC.
无菌（Sterility）
The sterility testing was performed by membrane filtration method described in CP<1101>, USP<71> and Ph. Eur. 2.6.1.
支原体（Mycoplasma）
Negative.
制剂（Formulation）
Supplied as 0.2 μm filtered solution in PBS, pH7.4 with protectants.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
For long term storage, the product should be stored at liquid state at -70°C.
This product is stable after storage at:
- 2-8°C for 12 months under sterile condition;
- -70°C for 5 years.
产品数据图
电泳（SDS-PAGE）
GMP Monoclonal Anti-Human CD3 Antibody (OKT3) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-HPLC
The purity of GMP Monoclonal Anti-Human CD3 Antibody (OKT3) (Cat. No. GMP-MC0323) was greater than 95% as determined by SEC-HPLC.
活性（Bioactivity）-Bioactivity CELL BASE
GMP Monoclonal Anti-Human CD3 Antibody (OKT3) (Cat. No. GMP-MC0323) stimulates secretion of IL-2 by PBMC stimulated with 10 ng/mL Monoclonal Anti-Human CD28 Antibody, Mouse IgG1. The typically EC50 for this effect is 0.11 ng/mL (QC tested).
Protocol
The activity of GMP Monoclonal Anti-Human CD3 Antibody (OKT3) (Cat. No. GMP-MC0323) was higher than other competing products.
稳定性（Stability）
The Cell based assay shows that GMP Monoclonal Anti-Human CD3 Antibody (OKT3) (Cat. No. GMP-MC0323) is stable at 37°C for 7 days.
The Cell based assay shows that GMP Monoclonal Anti-Human CD3 Antibody (OKT3) (Cat. No. GMP-MC0323) is stable after freezing and thawing 3 times.
The Cell based assay shows batch-to-batch consistency between Acro's GMP and PG CD3 Antibody.
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背景
CD3e molecule, epsilon is also known as CD3E, is a T-cell surface single-pass type I membrane glycoprotein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. CD3E gene has also been linked to a susceptibility to type I diabetes in women. CD3E has been shown to interact with TOP2B, CD3EAP and NCK2.
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