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产品描述（Product Details）
| Assay Type | Sandwich-ELISA |
| Analyte | AAV5 |
| Format | 96T |
| Regulatory Status | RUO |
| Sensitivity | < 1.70E+07 capsids/mL |
| Standard Curve Range | 1.09E+9 capsids/mL-1.70E+07 capsids/mL |
| Assay Time | 3 hr 20 min |
| Suitable Sample Type | For the detection and quantitative determination of AAV5 capsid titration in AAV gene therapy product preparation processing. |
| Sample volume | 100 uL |
组分（Materials Provided）
| ID | Components | Size |
| AAV05-C01 | Pre-coated Anti-AAV5 Antibody Microplate | 1 plate |
| AAV05-C02 | AAV5 Standard | 8.7E+10capsids |
| AAV05-C03 | Biotin-Anti-AAV5 Antibody | 20 μg |
| AAV05-C04 | Streptavidin-HRP | 50 μL |
| AAV05-C05 | 10xWashing Buffer | 50 mL |
| AAV05-C06 | 2xDilution Buffer | 50 mL |
| AAV05-C07 | Substrate Solution | 12 mL |
| AAV05-C08 | Stop Solution | 7 mL |
产品数据图
典型数据-Typical Data Please refer to Ds document for the assay protocol.
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
批内差异（Intra-Assay Statistics）
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision, Intra-Assay Precision CV＜10%.
批间差异（Inter-Assay Statistics）
Three samples of known concentration were tested in three separate assays to assess inter-assay precision, Inter-Assay Precision CV<10%.
回收率（Recovery）
Three AAV5 capsid with different concentrations were tested to calculate the recovery rate.
专属性（Specificity）
The specific binding of AAV-A005 to AAV1/2/3/8/9/dj serotypes was detected. The results showed that AAV-A005 only binds specifically to AAV5.
The specific binding of AAV-A005 to native/denatured AAV5 was detected. The results showed that AAV-A005 only binds specifically to native AAV5. (Denatured AAV5: 95℃ for 10min)
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背景
The adeno-associated virus (AAV) is a small (25 nm), non-enveloped virus of the parvoviridae family, including 12 different AAV serotypes. In the parvoviridae family it belongs to the genus dependoparvovirus, because it needs the presence of a helper virus for replication and assembly. The icosahedral AAV capsid composed of the capsid proteins VP1, VP2 and VP3 contains a linear, single-stranded DNA genome of 4.7 kb.
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