"膜杰作"——四次跨膜蛋白Claudin18.2（HEK293 表达）
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以下ELISA/SPR/BLI/FACS数据均可免费提供内部实验操作方法以供参考，点击申请Protocol即可获取。
高免疫原性
高免疫原性：VLP平台提高了靶抗原的丰度，增强了免疫效果
多领域应用：可用于免疫/ELISA/SPR/BLI/细胞实验/CAR阳性率检测
高稳定性与活性：提供稳定且高活性的Claudin 18.2蛋白
适用于免疫/ELISA/SPR/BLI/细胞实验/CAR阳性率检测
高活性验证：经FACS验证，Claudin 18.2稳定细胞株与抗Claudin 18.2抗体特异性结
可用于免疫/细胞水平的抗体筛选
Immobilized Human Claudin-18.2 Full Length Protein-VLP (Cat. No. CL2-H52P7) at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Chimeric Claudin-18.2 Antibody, Human IgG1 with a linear range of 0.2-3 ng/mL (QC tested).
Protocol
Human Claudin-18.2 Full Length Protein-VLP (Cat. No. CL2-H52P7) captured on CM5 Chip via Anti-Claudin-18.2 antibody can bind Anti-Claudin-18.2 antibody with an affinity constant of 0.374 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
结果表明，Claudin18.2-VLP 蛋白（Cat. No. CL2-HF218）可与Claudin18.2-CAR-293细胞特异性结合。
2e5 of Anti-Claudin-18.2 CAR-293 cells were stained with 100 μL of 3 μg/mL of Fluorescent Human Claudin-18.2 Full Length Protein-VLP (Cat. No.CL2-HF218) and negative control protein respectively, FITC signals was used to evaluate the binding activity (Routinely tested).
Protocol
Immobilized Human Claudin-18.2, His,Twin-Strep Tag (Cat. No. CL2-H5587) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Chimeric Claudin-18.2 Antibody, Human IgG1 with a linear range of 0.1-2 ng/mL (QC tested).
Protocol
Anti-Claudin-18.2 mAb captured on CM5 chip via anti-Human IgG (Fc) antibody can bind Human Claudin-18.2, His,Twin-Strep Tag (Cat. No. CL2-H5587) with an affinity constant of 6.72 nM as determined in a SPR assay (in presence of DDM and CHS) (Biacore T200) (Routinely tested).
Protocol
Immobilized Biotinylated Human Claudin-18.2 Protein, His,Avitag&Flag Tag (Cat. No. CL2-H85D4) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Monoclonal Anti-Chimeric Claudin-18.2 Antibody, Human IgG1 with a linear range of 0.1-2 ng/mL (QC tested).
Protocol
Monoclonal Anti-Chimeric Claudin-18.2 Antibody, Human IgG1 captured on Protein A Chip can bind Human / Cynomolgus Claudin-18.2, His,Twin-Strep Tag (Cat. No. CL2-H5586) with an affinity constant of 12.6 nM as determined in SPR assay (Biacore 8K) (Routinely tested).
Protocol
FACS analysis of cell surface expression of Claudin-18.2 on HEK293/Human Claudin-18.2 Stable Cell Line(Cat. No. CHEK-ATP033). 0.2 million of untransfected HEK293 cells (grey line ) and HEK293/Human Claudin-18.2 stable cells (red line ) were stained with 100 µL of the diluted solution of anti-Claudin-18.2 antibody, Human IgG1 ,washed and then followed by PE anti-Human IgG1 Fc antibody and analyzed with flow cytometry .
Protocol
临床药物信息
相关参考文献
Jumper J, et al. Highly accurate protein structure prediction with AlphaFold. Nature (2021)
Furuse M, et al. Claudin-1 and -2: novel integral membrane proteins localizing at tight junctions with no sequence similarity to occludin[J]. J Cell Biol（1998）
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