收藏产品 产品信息
优势特色（Features）
- Designed under ISO 9001:2015 and ISO 13485:2016
- Manufactured and QC tested under a GMP compliance factory
- FDA DMF filed
- Animal-Free materials
- Beta-lactam materials free
- Batch-to-batch consistency
- Stringent quality control tests
- No animal derived peptone and lactose used in production process.
产品描述（Product Details）
GMP Salt Active GENIUS™Nuclease is developed with ACRObiosystems' unique enzyme AI evolution platform based on wild-type Serratia marcescens endonuclease. It is capable of maintaining high specific activity to effectively degrade nuclei acids in any form across a broad salt concentration range (0-500 mM NaCl), especially high salt up to 500mM NaCl, which makes it suitable for nucleic acid removal in processes that of higher salt conditions. The enzyme is a non-specific nuclease with high specific activity, degrading single- and double-stranded nucleic acids in any form (single stranded, double stranded, linear, circular, and supercoiled). It hydrolyzes internal phosphodiester bonds present between the nucleotides to 5’- phosphorylated oligonucleotides of 3-5 bases in length.
应用说明（Application）
Elimination of nucleic acids from biologics, suitable for samples with 0-500 mM salt in presence of 5-10 mM Mg++
• clinical viral vaccine production
• clinical viral vector production for cell and gene therapy (CGT)
• Other clinical development and production uses
纯度（Purity）
>95% as determined by SDS-PAGE.
>95% as determined by SEC-HPLC.
酶活（Enzyme Activity）
＞250 U/μL
宿主蛋白残留（Host Cell Protein）
<0.05 ng/µg of protein tested by ELISA.
蛋白酶活性（Protease Activity）
Negative.
无菌（Sterility）
The sterility testing was performed by membrane filtration method described in CP<1101>, USP<71> and Eur. Ph. 2.6.1.
支原体（Mycoplasma）
Negative.
内毒素（Endotoxin）
Less than 10 EU/mg by the LAL method.
制剂（Formulation）
Supplied as 0.2 μm filtered solution in 25 mM Tris, 500 mM NaCl, 5 mM MgCl2, pH7.5.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
This product is stable after storage at:
- The product MUST be stored at -20°C or lower upon receipt;
- -20°C for 5 years under sterile conditions.
产品数据图
电泳（SDS-PAGE）
The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-HPLC
The purity of GMP Salt Active GENIUSNuclease (Cat. No. GMP-NUES13) was greater than 95% as determined by SEC-HPLC.
活性（Bioactivity）
Specific activity for GMP Salt Active GENIUS™Nuclease is measured under standard assay conditions. The specific activity of GMP GENIUS Nuclease is >4.3E+05 unit/mg at 500 mM salt concentration (QC tested). One unit will digest sonicated salmon sperm DNA to acid-soluble oligonucleotides equivalent to a ΔA260 of 1.0 in 30 min at pH 8.0 at 37 °C, which corresponds approximately to complete digestion of 37 μg DNA.
GMP GENIUS Nuclease shows high specific activity.
The effect of Mg++ concentrations on Salt Active GENIUS™Nuclease activity. The Nuclease requires 5 mM Mg++ cations for optimal activity.
稳定性（Stability）
The specific activity shows that GMP Salt Active GENIUS™Nuclease (Cat. No. GMP-NUSE13) is stable in different batches.
The specific activity shows that GMP Salt Active GENIUS™Nuclease (Cat. No. GMP-NUSE13) is stable at 37℃ for 21 days.
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