收藏产品 产品信息
优势特色（Features）
High cleavage activity in different cell line
High purity (＞95% SDS-PAGE / SEC-MALS)
Animal-Free materials
Batch-to-batch consistency
Stringent quality control tests
产品描述（Product Details）
GENPower™ NLS-Cas9 Nuclease is a recombinant Streptococcus pyogenes Cas9 protein purified from Escherichia coli for CRISPR-based genome editing. The introduction of nuclear localization signals (NLS) can help Cas9 enter the nucleus, increasing the chance of genomic DNA cleavage.
浓度（Concentration）
10 mg/mL
纯度（Purity）
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
宿主蛋白残留（Host Cell Protein）
≤10 ng/mg of protein tested by ELISA.
宿主核酸残留（Host Cell DNA）
≤1 ng/mg of protein tested by qPCR.
无菌（Sterility）
Negative
支原体（Mycoplasma）
Negative.
内毒素（Endotoxin）
Less than 0.01 EU per μg by the LAL method.
制剂（Formulation）
Supplied as 0.2 μm filtered solution in 20 mM Tris, 300 mM NaCl, 0.1 mM EDTA, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
This product is stable after storage at:
- The product MUST be stored at -20°C or lower upon receipt;
- -20°C for 18 months under sterile conditions.
产品数据图
电泳（SDS-PAGE）
The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of GENPower™ NLS-Cas9 Nuclease, premium grade (Cat. No. CA9-S5149) is more than 95% and the molecular weight of this protein is around 140-180 kDa verified by SEC-MALS.
Report
活性（Bioactivity）
GENPower™ NLS-Cas9 Nuclease, premium grade is evaluated in an in vitro DNA cleavage assay on a DNA fragment containing the target sequence. The activity of the GENPower™ NLS-Cas9 Nuclease, premium grade is greater than 90% (QC tested).
The cleavage activity of GENPower™ NLS-Cas9 Nuclease, premium grade in vitro.
The cleavage activity of GENPower™ NLS-Cas9 Nuclease, premium grade in cell line.
The cleavage activity of GENPower™ NLS-Cas9 Nuclease, premium grade in human primary T cells.
The knockout efficiency for B2M in primary T cell was measured by Flow Cytometry.
The knockout efficiency for TRAC in Jurkat cell was measured by Flow Cytometry.
The knockout efficiency for B2M in Jurkat cell was measured by Flow Cytometry.
稳定性（Stability）
The bioactivity based assay shows batch-to-batch consistency between Acro's GMP and PG Cas9.
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