收藏产品 产品信息
分子别名（Synonym）
CD71,TFRC,TFR,sTfR,TfR1,Trfr,T9,p90
表达区间及表达系统（Source）
Biotinylated Human Transferrin R, Avitag,His Tag (TFR-H82E5) is expressed from human 293 cells (HEK293). It contains AA Cys 89 - Phe 760 (Accession # P02786-1).
Predicted N-terminus: Gly
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 78.7 kDa. The protein migrates as 90-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
产品数据图
电泳（SDS-PAGE）
Biotinylated Human Transferrin R, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Biotinylated Human Transferrin R, Avitag,His Tag (Cat. No. TFR-H82E5) is more than 90% and the molecular weight of this protein is around 160-180 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human Transferrin Protein, His Tag (Cat. No. TRN-H52H3) at 2 μg/mL (100 μL/well) can bind Biotinylated Human Transferrin R, Avitag,His Tag (Cat. No. TFR-H82E5) with a linear range of 1.2-9.7 ng/mL (QC tested).
Protocol
活性（Bioactivity）-SPR
Brain-penetrant, anti-amyloid, bispecific antibody immobilized on CM5 Chip can bind Biotinylated Human Transferrin R, Avitag,His Tag (Cat. No. TFR-H82E5) with an affinity constant of 29.2 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
产品评论 发表评论
背景
CD antigen CD71 is also known as Transferrin receptor protein 1, TfR, sTfR, p90, TfR1, Trfr, which belongs to the peptidase M28 family and M28B subfamily. CD71 /TFR contains one PA (protease associated) domain. CD71 / TfR1 is required for iron delivery from transferrin to cells. CD71 is a potential new target in cases of human leukomia & lymphoma. CD71 /TFRC / TfR has been shown to interact with GABARAP and HFE.
靶点信息 
数据表和文档 
联系我们 
项目合作