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分子别名（Synonym）
C7orf15,C7orf15MGC138295,CD112R,MGC104322,MGC138297,MGC2463,PVRIG,CD112 receptor
表达区间及表达系统（Source）
Biotinylated Human PVRIG, Fc,Avitag (PVG-H82F4) is expressed from human 293 cells (HEK293). It contains AA Thr 41 - Asp 171 (Accession # Q6DKI7-1).
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 41.9 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
Biotin
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
数据展示
电泳（SDS-PAGE）
Biotinylated Human PVRIG, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Biotinylated Human PVRIG, Fc,Avitag (Cat. No. PVG-H82F4) is more than 90% and the molecular weight of this protein is around 82-110 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Biotinylated Human PVRIG, Fc,Avitag (Cat. No. PVG-H82F4) at 5 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human Nectin-2, Mouse IgG2a Fc Tag (Cat. No. CD2-H5257) with a linear range of 0.1-3 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Human PVRIG, Fc,Avitag (Cat. No. PVG-H82F4) at 5 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human Nectin-2, His Tag (Cat. No. PV2-H52E2) with a linear range of 0.02-0.313 μg/mL (Routinely tested).
Protocol
Immobilized Human Nectin-2, His Tag (Cat. No. PV2-H52E2) at 10 μg/mL (100 μL/well) can bind Biotinylated Human PVRIG, Fc,Avitag (Cat. No. PVG-H82F4) with a linear range of 0.005-0.313 μg/mL (Routinely tested).
Protocol
Immobilized Human Nectin-2, Fc Tag (Cat. No. PV2-H5253) at 10 μg/mL (100 μL/well) can bind Biotinylated Human PVRIG, Fc,Avitag (Cat. No. PVG-H82F4) with a linear range of 0.005-0.313 μg/mL (Routinely tested).
Protocol
Immobilized Human Nectin-2, Mouse IgG2a Fc Tag (Cat. No. CD2-H5257) at 10 μg/mL (100 μL/well) can bind Biotinylated Human PVRIG, Fc,Avitag (Cat. No. PVG-H82F4) with a linear range of 0.01-0.313 μg/mL (Routinely tested).
Protocol
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