收藏产品 产品信息
分子别名（Synonym）
MSLN,Mesothelin,MPF
表达区间及表达系统（Source）
Alexa Fluor 647-Labeled Human Mesothelin (296-580), His Tag (MSN-HA2H5) is produced via conjugation of AF647 to Human Mesothelin (296-580), His Tag with a new generation site-specific technology under Star Staining labeling platform. Human Mesothelin (296-580), His Tag is expressed from human 293 cells (HEK293). It contains AA Glu 296 - Gly 580 (Accession # AAH09272.1).
Predicted N-terminus: Glu 296
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 49.1 kDa.
偶联（Conjugate）
AF647
Excitation Wavelength: 640 nm
Emission Wavelength: 672 nm
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
产品数据图
活性（Bioactivity）-FACS
The activity of Alexa Fluor 647-Labeled Human Mesothelin (296-580), His Tag (Cat. No. MSN-HA2H5) was higher than other Competitor R.
Protocol
CAR阳性表达率检测（Evaluation of CAR expression）
FACS Analysis of Anti-Mesothelin CAR Expression
5e5 of anti-MSLN CAR-293 cells were stained with 100 μL of 1 µg/mL of Alexa Fluor 647-Labeled Human Mesothelin (296-580), His Tag (Cat. No. MSN-HA2H5) and negative control protein respectively (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). AF647 signal was used to evaluate the binding activity (QC tested).
FACS Analysis of Non-specific binding to PBMCs
5e5 of PBMCs were stained with Alexa Fluor 647-Labeled Human Mesothelin (296-580), His Tag (Cat. No. MSN-HA2H5) and anti-CD3 antibody, washed and then analyzed with FACS. FITC signal was used to evaluate the expression of CD3+ T cells in PBMCs, and AF647 signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).
产品评论 发表评论
背景
Mesothelin (MSLN) is also known as CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, which belongs to the mesothelin family. Mesothelin / MSLN can be proteolytically cleaved into the following two chains by a furin-like convertase: Megakaryocyte-potentiating factor (MPF) and the cleaved form of mesothelin. Both MPF and the cleaved form of mesothelin are N-glycosylated. Mesothelin / MSLN can interacts with MUC16. The membrane-anchored forms of MSLN may play a role in cellular adhesion. MPF potentiates megakaryocyte colony formation in vitro.
靶点信息 
数据表和文档 
联系我们 
项目合作