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分子别名（Synonym）
DLL4,Delta4
表达区间及表达系统（Source）
Human DLL4 Protein, Fc Tag, premium grade (Flagship) (DL4-H5255) is expressed from human 293 cells (HEK293). It contains AA Ser 27 - Pro 524 (Accession # NP_061947.1).
Predicted N-terminus: Ser 27
This Flagship Premium Grade product is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a GMP equivalent GMP-DL4H27, GMP Human DLL4 Protein, Fc Tag (Flagship), which has an identical performance profile, thereby ensuring a seamless transition.
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 80.7 kDa. The protein migrates as 90 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
Unconjugated
内毒素（Endotoxin）
Less than 0.01 EU per μg by the LAL method.
蛋白A残留（Protein A）
5 ppm of protein tested by ELISA.
宿主蛋白残留（Host Cell Protein）
0.5 ng/µg of protein tested by ELISA.
宿主核酸残留（Host Cell DNA）
0.02 ng/μg of protein tested by qPCR.
无菌（Sterility）
Negative
支原体（Mycoplasma）
Negative.
纯度（Purity）
95% as determined by SDS-PAGE.
90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
产品展示
电泳（SDS-PAGE）
Human DLL4 Protein, Fc Tag, premium grade (Flagship) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human DLL4 Protein, Fc Tag, premium grade (Flagship) (Cat. No. DL4-H5255) is more than 90% and the molecular weight of this protein is around 160-200 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-CELL BASE
Human DLL4 Protein, Fc Tag, premium grade (Flagship) (Cat. No. DL4-H5255) stimulates Human Notch1 (Luc) HeLa Reporter Cell. The typically EC50 for this effect is 597 ng/mL (QC tested).
Protocol
活性（Bioactivity）-SPR
Human DLL4 Protein, Fc Tag, premium grade (Flagship) (Cat. No. DL4-H5255) captured on Protein A Chip can bind Human NOTCH1 Protein, His Tag, premium grade (Cat. No. NO1-H52H3) with an affinity constant between 1.00 nM - 150 nM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
应用数据（Application Data）
CD34+ CD45+ hematopoietic cells were seeded on a Human DLL4 Protein, Fc Tag, premium grade (Flagship) (Cat. No. DL4-H5255) coating plate and differentiated for 14 days, then flow cytometry was used to detect the expression of T-cell progenitor markers, CD7 and CD5, in FSC/SSClow gate. Human DLL4 Protein, Fc Tag, premium grade (Flagship) (Cat. No. DL4-H5255) together with other factors could induce the formation of CD7+ and CD7+ CD5+ T-cell progenitors. However, the cells cultured without Human DLL4 Protein, Fc Tag, premium grade (Flagship) (Cat. No. DL4-H5255) expressed neither CD5 nor CD7 (Routinely tested).
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