产品详情
分子别名(Synonym)
BLR2, CC-CKR-7, CCR-7, CD197, CDw197, CMKBR7, EBI1
表达区间及表达系统(Source)
Human CCR7 Full Length Protein, His,Flag Tag (CC7-H52D3) is expressed from human 293 cells (HEK293). It contains AA Gln 25 - Pro 378 (Accession # P32248).
Predicted N-terminus: Gln 25
Request for sequence蛋白结构(Molecular Characterization)
This protein carries a polyhistidine tag at the C-terminus, followed by a Flag tag. The protein has a calculated MW of 58.9 kDa.
偶联(Conjugate)
Unconjugated
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
纯度(Purity)
85% as determined by SDS-PAGE.
制剂(Formulation)
This product is not suitable for cell based experiments due to cytotoxicity of detergent.
Detergent buffer is INDISPENSABLE to keep membrane protein soluble and active, under no circumstances should you remove detergent.
Detergent buffer is sold separately and not included in protein, and please contact us if you need the buffer.
If glycerol is not compatible with your application, remove glycerol just before the immediate experiment, and NEVER store glycerol-free protein solution.Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, Buffer B, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
运输(Shipping)
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储(Storage)
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
质量管理控制体系(QMS)
- *The detergent Buffer B (Cat. No. LG-13) is sold separately and not included in protein, you can follow this link for product information.
产品展示
活性(Bioactivity)-ELISA
Immobilized Human CCR7 Full Length Protein, His,Flag Tag (Cat. No. CC7-H52D3) at 1 μg/mL (100 μL/well) can bind Anti-CCR7 Antibody, Human IgG1 with a linear range of 0.2-8 ng/mL (QC tested).
Protocol
活性(Bioactivity)-SPR
Anti-CCR7 Antibody, Human IgG1 captured on Protein A Chip can bind Human CCR7 Full Length Protein, His,Flag Tag (Cat. No. CC7-H52D3) with an affinity constant of 121 nM as determined in a SPR assay (in presence of DDM and CHS) (Biacore 8K) (Routinely tested).
Protocol
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