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分子别名（Synonym）
B7-H7,HHLA2,B7 Homolog 7
表达区间及表达系统（Source）
Human B7-H7, His Tag (B77-H52H6) is expressed from human 293 cells (HEK293). It contains AA Ile 23 - Asn 344 (Accession # Q9UM44-1).
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 39.0 kDa. The protein migrates as 50-66 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
Unconjugated
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
数据展示
电泳（SDS-PAGE）
Human B7-H7, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human B7-H7, His Tag (Cat. No. B77-H52H6) is more than 90% and the molecular weight of this protein is around 47-65 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human CD28H, Fc Tag (Cat. No. CDH-H5251) at 5 μg/mL (100 μL/well) can bind Human B7-H7, His Tag (Cat. No. B77-H52H6) with a linear range of 0.078-2.5 μg/mL (QC tested).
Protocol
Immobilized Human B7-H7, His Tag (Cat. No. B77-H52H6) at 5 μg/mL (100 μL/well) can bind Human CD28H, Fc Tag (Cat. No. CDH-H5251) with a linear range of 10-156 ng/mL (Routinely tested).
Protocol
Immobilized Biotinylated Human KIR3DL3 / CD158z Protein, Fc,Avitag (Cat. No. KI3-H82F3) at 2 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human B7-H7, His Tag (Cat. No. B77-H52H6) with a linear range of 0.02-5 μg/mL (Routinely tested).
Protocol
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