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分子别名（Synonym）
SNCA,NACP,PARK1,alpha-Synuclein
表达区间及表达系统（Source）
Human Alpha-Synuclein, Fc Tag (ALN-H5253) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 140 (Accession # P37840-1).
Predicted N-terminus: Met 1
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus
The protein has a calculated MW of 40.9 kDa. The protein migrates as 47-52 kDa and 53-56 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
产品数据图
电泳（SDS-PAGE）
Human Alpha-Synuclein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human Alpha-Synuclein, Fc Tag (Cat. No. ALN-H5253) is more than 90% and the molecular weight of this protein is around 75-95 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human Alpha-Synuclein, Fc Tag (Cat. No. ALN-H5253) at 1 μg/mL (100 μL/well) can bind Anti-Synucelin alpha/beta Monoclonal Antibody with a linear range of 0.031-1 μg/mL (QC tested).
Protocol
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背景
Alpha-synuclein is a neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. It acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DJC5. Abnormalities in alpha-synuclein are implicated in the pathogenesis of Parkinson's disease (PD). Alpha-synuclein is present in Lewy-bodies, the neuropathological hallmark of PD, and the protein and its aggregation have been widely linked to neurotoxic pathways that ultimately lead to neurodegeneration.
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