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Human Alpha-Synuclein Pre-formed Fibrils Protein, His Tag

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价格
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ALN-H51H4-100ug
¥3500.00
ALN-H51H4-500ug
¥13100.00
ALN-H51H4-1mg
¥21000.00
合计0件 产品金额¥ 0
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产品详情

  • 分子别名（Synonym）

    SNCA,NACP,PARK1,alpha-Synuclein

  • 表达区间及表达系统（Source）

    Human Alpha-Synuclein Pre-formed Fibrils, His Tag (ALN-H51H4) is expressed from E. coli cells. It contains AA Met 1 - Ala 140 (Accession # P37840-1).

    Predicted N-terminus: Met 1

    Request for sequence

  • 蛋白结构（Molecular Characterization）

    Alpha-Synuclein Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 16.3 kDa.

  • 偶联（Conjugate）

    Unconjugated

  • 内毒素（Endotoxin）

    Less than 1.0 EU per μg by the LAL method.

  • 纯度（Purity）

    90% as determined by SDS-PAGE.

  • 制剂（Formulation）

    Supplied as 0.2 μm filtered solution in PBS, pH7.4.

    Contact us for customized product form or formulation.

  • 运输（Shipping）

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • 存储（Storage）

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product should be stored at -70°C or room temperature for short storage. Do not store fibrils on ice or at 4°C;
    2. The unsonicated fibril is validated to be stable after storage at -70°C for 1 year under sterile conditions;
    3. The sonicated fibril should be stored at -70°C for not more than 8 weeks.

  • 质量管理控制体系（QMS）

    1. 质量管理体系（ISO, GMP）
    2. 质量优势
    3. 质控流程

产品展示

  • 电泳（SDS-PAGE）

    Alpha-Synuclein SDS-PAGE

    Alpha-Synuclein monomer on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

  • 活性（Bioactivity）-ThT Assay

    Alpha-Synuclein THT

    Thioflavin T is a sensitive fluorescence reporter of fibrils formulation that binds to beta sheet-rich structures. The alpha synuclein pre-formed fibrils (Cat. No. ALN-H51H4) is able to induce the aggregation of alpha synuclein monomers (Cat. No. ALN-H52H8) (Routinely tested).

    Protocol

  • 电镜（Electron Microscope）

    Alpha-Synuclein ELECTRON MICROSCOPE

    Transmission electron microscopy (TEM) of alpha-synuclein preformed fibrils (Cat. No. ALN-H51H4). Fibril structure is visible on negative stain TEM images of ALN-H51H4 (Routinely tested).

  • 活性（Bioactivity）-CELL BASE

    Alpha-Synuclein CELL

    HEK293/Human Alpha-Synuclein (GFP) Stable Cell Line (Cat. No. CHEK-ATP085) were transduced with Human Alpha-Synuclein Pre-formed Fibrils, His Tag (Cat. No. ALN-H51H4) and Human Alpha-Synuclein, His Tag (Cat. No. ALN-H52H8) respectively. The fluorescence of GFP-Alpha-Synuclein (Green) and DAPI (Blue) were detected by confocal microscope. A. Lipo2000 transduction. B. Lipo2000 and Human Alpha-Synuclein, His Tag transduction. C. Lipo2000 and Human Alpha-Synuclein Pre-formed Fibrils, His Tag transduction. Scale bars, 50 μm (Routinely tested).

    Protocol

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背景介绍

Alpha-synuclein is a neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. It acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DJC5. Abnormalities in alpha-synuclein are implicated in the pathogenesis of Parkinson's disease (PD). Alpha-synuclein is present in Lewy-bodies, the neuropathological hallmark of PD, and the protein and its aggregation have been widely linked to neurotoxic pathways that ultimately lead to neurodegeneration.

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药物研发进展
  • 英文全称：

    Synuclein alpha

  • 中文全称：

    α-突触核蛋白

  • 种类：

  • 上市药物数量：

    0 详情

  • 临床药物数量：

    23 详情

  • 最高研发阶段：

    临床三期

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