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分子别名（Synonym）
ACE-2,ACEH,ACE2
表达区间及表达系统（Source）
Human ACE2, His Tag (AC2-H52H4) is expressed from human 293 cells (HEK293).
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 85.5 kDa. The protein migrates as 90-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
Unconjugated
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Supplied as 0.2 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
质量管理控制体系（QMS）
数据展示
电泳（SDS-PAGE）
Human ACE2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human ACE2, His Tag (Cat. No. AC2-H52H4) is more than 85% and the molecular weight of this protein is around 170-220 kDa verified by SEC-MALS.
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活性（Bioactivity）-ELISA
Immobilized Human ACE2, His Tag (Cat. No. AC2-H52H4) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 S protein RBD, Fc Tag (Cat. No. SPD-C5255) with a linear range of 0.1-0.8 ng/mL (QC tested).
Protocol
Immobilized Human ACE2, His Tag (Cat. No. AC2-H52H4) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 S1 protein, Fc Tag (Cat. No. S1N-C5255) with a linear range of 0.4-6 ng/mL (Routinely tested).
Protocol
Immobilized SARS-CoV-2 S protein RBD, Fc Tag (Cat. No. SPD-C5255) at 2 μg/mL (100 μL/well) can bind Human ACE2, His Tag (Cat. No. AC2-H52H4) with a linear range of 0.02-0.16 μg/mL (Routinely tested).
Protocol
Immobilized SARS-CoV-2 S1 protein, Fc Tag (Cat. No. S1N-C5255) at 2 μg/mL (100 μL/well) can bind Human ACE2, His Tag (Cat. No. AC2-H52H4) with a linear range of 39-156 ng/mL (Routinely tested).
Protocol
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