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组分（Materials Provided）
IDComponentsSizeEP143-C01High-bind Plate1 plateEP143-C02Human TNFR115 μgEP143-C03Biotinylated Human TNF-alpha10 μgEP143-C04Anti-TNF-alpha Neutralizing Antibody20 μgEP143-C05Streptavidin-HRP10 μgEP143-C06Coating Buffer12 mLEP143-C0710xWashing Buffer50 mLEP143-C08Blocking Buffer50 mLEP143-C09Substrate Solution12 mLEP143-C10Stop Solution7 mL产品概述（Product Overview）
TNF-alpha [Biotinylated] : TNFR1 Inhibitor Screening ELISA Kit is based on a competition-ELISA method, is used to screening for inhibitors of human TNF-alpha binding to human TNFR1. The kit is designed to provide a reliable solution for the screening of antibodies and small molecule inhibitors in the early stages, as well as QC release, to help researchers quickly screen and validate drugs, effectively improving research and development efficiency. It can also be used as a universal detection tool to identify the ability of human TNF-alpha binding to human TNFR1.
重构方法（Reconstitution）
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存储（Storage）
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
质量管理控制体系（QMS）
产品展示
典型数据-Typical Data
Please refer to DS document for the assay protocol.
INHIBITION OF TNF-alpha [Biotinylated]: TNFR1 BINDING BY ANTI- TNF-alpha NEUTRALIZING ANTIBODY
Serial dilutions of Anti-TNF-alpha Neutralizing antibody (Catalog # EP143-C04) (1:1 serial dilution, from 10 μg/mL to 0.005μg/mL) was added into Biotinylated TNF-alpha: TNFR1 binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).
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