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HRSV Pre-Fusion glycoprotein F0 Specific ELISA Kit

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RAS-A205-96tests
¥8190.00
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  • 组分(Materials Provided)

    ID
    Components
    Size
    RAS205-C01
    Pre-coated Anti-Pre-Fusion glycoprotein F0 (RSV) Antibody Microplate
    1 plate
    RAS205-C02
    Pre-Fusion glycoprotein F0 (RSV) Standard
    15 μg
    RAS205-C03
    HRP-Anti-Pre-Fusion glycoprotein F0 (RSV) Antibody
    20 μg
    RAS205-C04
    10×Washing Buffer
    50 mL
    RAS205-C05
    2×Dilution Buffer
    50 mL
    RAS205-C06
    Substrate Solution
    12 mL
    RAS205-C07
    Stop Solution
    7 mL

  • 产品概述(Product Overview)

    Respiratory syncytial virus (RSV) is a highly contagious virus causing severe infection in infants and the elderly. Various approaches are being used to develop an effective RSV vaccine. The RSV fusion (F) subunit, particularly the cleaved trimeric pre-fusion F, is one of the most promising vaccine candidates under development.

    A rapid and effective assay kit detecting the levels of HRSV Pre-Fusion glycoprotein F0 is urgently needed to accelerate the development of RSV vaccines.

  • 应用说明(Application)

    The kit has been tested to specifically identify HRSV (A) and HRSV (B) Pre-Fusion glycoprotein. It was developed for the specific quantitative detection of HRSV Pre-Fusion glycoprotein in samples.

    It is for research use only.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

  • 存储(Storage)

    2-8℃

  • 原理(Assay Principles)

    This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of HRSV Pre-Fusion glycoprotein F0. The kit consists of Pre-coated Anti-Pre-Fusion glycoprotein F0 (RSV) Antibody Microplate and Pre-Fusion glycoprotein F0 (RSV) Standard,HRP-Anti-Pre-Fusion glycoprotein F0 (RSV) Antibody and buffers.

    Your experiment will include 5 simple steps:

    a) Bring all reagents and samples to room temperature (20℃-25℃) before use.

    b) Add your sample to the plate, take the Pre-Fusion glycoprotein F0 (RSV) as Control sample. The samples and Control sample are diluted by Dilution Buffer.

    c) Add the HRP-Anti-Pre-Fusion glycoprotein F0 (RSV) Antibody diluted by Dilution Buffer to the plate.

    d) Wash the plate and add TMB.

    e) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.

  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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  • 典型数据-Typical Data

    Please refer to DS document for the assay protocol.

     Pre-Fusion glycoprotein F0 TYPICAL DATA

    For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.

  •  Pre-Fusion glycoprotein F0 TYPICAL DATA

    The kit has been tested to specifically identify HRSV (A) and HRSV (B) Pre-Fusion glycoprotein F0, and do not identify HRSV (A) and HRSV (B) Post-Fusion glycoprotein F0.

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