HRSV Post-Fusion glycoprotein F0 Specific ELISA Kit (For Vaccine Development)

组分（Materials Provided）

产品概述（Product Overview）

Most in vitro RSV neutralizing antibodies in human sera are directed against the prefusion conformation, but due to its instability the prefusion conformation has a propensity to prematurely refold into the stable postfusion conformation, both in solution and on the surface of the virions.

An RSV F protein that has both high expression levels and maintains a stable prefusion conformation would, therefore, be a promising subunit vaccine candidate against RSV.

To facilitate the RSV-related research, drug trials and vaccine development,a rapid and effective assay kit detecting the specific levels of HRSV Post-Fusion glycoprotein F0 is urgently needed to accelerate the development of RSV vaccines.

应用说明（Application）

It was developed for the specific quantitative detection of HRSV Post-Fusion glycoprotein in samples.

It is for research use only.

重构方法（Reconstitution）

Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

存储（Storage）

2-8℃

原理（Assay Principles）

This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of Post-Fusion glycoprotein F0 (RSV). The kit consists of Pre-coated Anti-Post-Fusion glycoprotein F0 (RSV) Antibody Microplate and Post-Fusion glycoprotein F0 (RSV) Standard,HRP-Anti-RSV-F0 Antibody and buffers.

Your experiment will include 5 simple steps:

a) Bring all reagents and samples to room temperature (20℃-25℃) before use.

b) Add your sample to the plate, take the Post-Fusion glycoprotein F0 (RSV) as Control sample. The samples and Control sample are diluted by Dilution Buffer.

c) Add the HRP-Anti-RSV-F0 Antibody diluted by Dilution Buffer to the plate.

d) Wash the plate and add TMB.

e) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.

质量管理控制体系（QMS）

1. 质量管理体系（ISO, GMP）
2. 质量优势
3. 质控流程