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组分（Materials Provided）
IDComponentsSizeEP132-C01High-bind Plate1 plateEP132-C02Human IL-4 R alpha30 μgEP132-C03Human IL-420 μgEP132-C04Human IL-4-Biotin10 μgEP132-C05Streptavidin-HRP10 μgEP132-C06Coating Buffer12 mLEP132-C0710xWashing Buffer50 mLEP132-C08Blocking Buffer50 mLEP132-C09Substrate Solution12 mLEP132-C10Stop Solution7 mL产品概述（Product Overview）
IL-4 [Biotinylated] : IL-4Rα Inhibitor Screening ELISA Kit is based on a competition-ELISA method, is used to screening for inhibitors of human IL-4 binding to human IL-4Rα. The kit is designed to provide a reliable solution for the screening of antibodies and small molecule inhibitors in the early stages, as well as QC release, to help researchers quickly screen and validate drugs, effectively improving research and development efficiency. It can also be used as a universal detection tool to identify the ability of human IL-4 binding to human IL-4Rα.
重构方法（Reconstitution）
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存储（Storage）
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
质量管理控制体系（QMS）
数据展示
典型数据-Typical Data
Please refer to DS document for the assay protocol.
INHIBITION OF IL-4 [BIOTINYLATED]: IL-4 R ALPHA BINDING BY Human IL-4
Serial dilutions of Human IL-4(Catalog # EP132-C03) (1:1 serial dilution, from 5 μg/mL to 0.01 μg/mL (295.86-0.58 nM)) was added into IL-4 R alpha: IL-4-Biotin binding reactions. The assay was performed according to the above-described protocol. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).
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