ELISA Buffer Set (Phosphate system, 96T)

背景（Background）

This ELISA Buffer Set-96T contains a High-bind 96well plate, coating buffer, washing buffer, blocking buffer, substrate solution and stop solution. The ELISA Buffer Set-96T can be quickly applied to the ELISA experiment and save your time to prepare buffers. This ELISA Buffer Set-96T is sterile-filtered.

应用说明（Application）

Coating buffer: Use coating buffer to dilute the sample you will coat, add 100μL/well to high-bind plate.

Washing buffer: Dilute the washing buffer to 1x with distilled water before experiment, add 300μL/well for washing the coated plate.

Blocking buffer: Dilute the blocking buffer to 1x with distilled water before experiment, add 300μL/well for blocking the coated plate. The samples and detection antibodies can be prepared by 1x blocking buffer which should be 4 times dilution.

Substrate solution: Add 100μL/well to plate.

Stop solution: Add 50μL/well to plate.

It is for research use only.

存储（Storage）

Store this set at 2-8°C, and protect from light. If precipitation occurs, filter to clarify before use.

原理（Assay Principles）

This ELISA Buffer Set-96T contains a High-bind 96well plate, coating buffer, washing buffer, blocking buffer, substrate solution and stop solution. The ELISA Buffer Set-96T can be quickly applied to the ELISA experiment and save your time to prepare buffers. This ELISA Buffer Set-96T is sterile-filtered.