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- Genetically modified cell lines best reflect MOA (Mechanism of Action)
- Higher activity and larger assay window for robust and reproducible cell-based bioassay
- Comprehensive application data to support assay development and validation
- Full tracible record, stringent quality control and validated cell passage stability
- Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
- Global commercial license assistance whenever regulatory filing is required
描述（Description）
The CHO/ Human PD-L1 Stable Cell Line was engineered to express full length human PD-L1 receptor (Gene ID: 29126), with different levels of PD-L1 expression (High, Medium, Low), which can be used to mimic cancer target cells with various PD-L1 expression levels. When co-cultured with Human 4-1BB HEK293 Reporter Cell and anti-human 4-1BB/PD-L1 BsAb, the anti-human 4-1BB/PD-L1 BsAb can be crosslinked, thereby strengthening 4-1BB pathway-activated luminescence.
应用说明（Application）
• Useful for cell-based PD-L1 binding assay
• Useful for PD-L1-mediated crosslinking in reporter gene assay
生长特性（Growth Properties）
Adherent
筛选标记（Selection Marker）
Puromycin (2 μg/mL)
培养基（Complete Growth Medium）
F-12K+ 10% FBS
冻存液（Freeze Medium）
Serum-free cell cryopreservation medium
装量（Quantity）
1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
存储（Storage）
Frozen in liquid nitrogen.
支原体检测（Mycoplasma Testing）
Negative
无菌检测（Sterility Testing）
Negative
使用说明（Instructions for Use）
See data sheet for detailed culturing and assay protocol.
产品数据图
Receptor Assay
Expression analysis of human PD-L1 on CHO/ Human PD-L1 Stable Cell Line by FACS.
Cell surface staining using PE-labeled anti-human PD-L1 antibody was performed on CHO/Human PD-L1 Stable Cell Line with different expression levels: CHO/Human PD-L1 Stable Cell Line (Low Expression); CHO/Human PD-L1 Stable Cell Line (Medium Expression); CHO/Human PD-L1 Stable Cell Line (High Expression).
Protocol
Application
Bioactivity analysis of anti-human 4-1BB/PD-L1 BsAb through CHO/Human PD-L1 Stable Cell Line (Medium Expression) crosslinking to test whether in a PD-L1-dependent manner to strengthen the agonistic activity.
The EC50 of anti-human 4-1BB/PD-L1 BsAb antibody is approximately 0.033 μM through CHO/Human PD-L1 Stable Cell Line (Medium Expression) crosslinking.
Protocol
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背景
Programmed cell death 1 ligand 1 (PDL1) is also known as B7-H, B7H1, MGC142294, MGC142296, PD-L1, PDCD1L1 and PDCD1LG1,which is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses.PDL1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. This protein is broadly expressed in the majority of peripheral tissues as well as hematopoietic cells. Interaction between PDL1 and its receptors belonging to the CD28 family of molecules provide both stimulatory and inhibitory signals in regulating T cell activation and tolerance. PDL1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression.
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