积分兑换
收藏产品 产品信息
- Genetically modified cell lines best reflect MOA (Mechanism of Action)
- Higher activity and larger assay window for robust and reproducible cell-based bioassay
- Comprehensive application data to support assay development and validation
- Full tracible record, stringent quality control and validated cell passage stability
- Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
- Global commercial license assistance whenever regulatory filing is required
描述（Description）
The HEK293/Human NAPI-IIb Stable Cell Line was engineered to express the receptor full length human NAPI-IIb (Gene ID: 10568), used to mimic cancer target cells. Surface expression of Human NAPI-IIb was confirmed by flow cytometry.
应用说明（Application）
• Useful for cell-based NAPI-IIb binding assay
生长特性（Growth Properties）
Adherent
筛选标记（Selection Marker）
Puromycin (2 μg/mL)
培养基（Complete Growth Medium）
DMEM + 10% FBS
冻存液（Freeze Medium）
Serum-free cell cryopreservation medium
装量（Quantity）
1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
存储（Storage）
Frozen in liquid nitrogen.
支原体检测（Mycoplasma Testing）
Negative
无菌检测（Sterility Testing）
Negative
使用说明（Instructions for Use）
See data sheet for detailed culturing and assay protocol.
产品数据图
Receptor Assay
Expression analysis of human NAPI-IIb on HEK293/Human NAPI-IIb Stable Cell Line by FACS.
Cell surface staining was performed on HEK293/Human NAPI-IIb Stable Cell Line or negative control cell using anti-human NAPI-IIb antibody followed by PE anti-human IgG Fc antibody after fixation with 4% paraformaldehyde.
Protocol
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背景
NAPI-IIb, NaPi-2b, Npt2b, or SLC34A2 enables sodium:phosphate symporter activity. Acts upstream of or within in utero embryonic development and phosphate ion transport. Located in apical plasma membrane and brush border. Is expressed in several structures, including egg cylinder; genitourinary system; small intestine; and tooth. Human ortholog(s) of this gene implicated in pulmonary alveolar microlithiasis. Orthologous to human SLC34A2 (solute carrier family 34 member 2). [provided by Alliance of Genome Resources, Apr 2022]
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