收藏产品 产品信息
- Genetically modified cell lines best reflect MOA (Mechanism of Action)
- Higher activity and larger assay window for robust and reproducible cell-based bioassay
- Comprehensive application data to support assay development and validation
- Full tracible record, stringent quality control and validated cell passage stability
- Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
- Global commercial license assistance whenever regulatory filing is required
描述（Description）
HEK293/Human Anti-CD19 Stable Cell Line
应用说明（Application）
• Binding assay by FACS and cell based ELISA
生长特性（Growth Properties）
Adherent
筛选标记（Selection Marker）
Puromycin (5 μg/mL)
培养基（Complete Growth Medium）
DMEM medium + 10% FBS
冻存液（Freeze Medium）
10% DMSO + 90% FBS
装量（Quantity）
1 vial contains at least 5x10^6 cells in 1 ml of 10% DMSO in FBS.
存储（Storage）
Frozen in liquid nitrogen.
支原体检测（Mycoplasma Testing）
Negative
无菌检测（Sterility Testing）
Negative
使用说明（Instructions for Use）
See data sheet for detailed culturing and assay protocol.
产品数据图
Receptor Assay
FACS assay shows that PE-Labeled Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 can bind to HEK293/Human Anti-CD19 Stable Cell Line. HEK293/Human Anti-CD19 Stable Cell Line was red line, Negative control HEK293 cells was grey line(QC tested).
Protocol
FACS assay shows that PE-Labeled Human CD19 (20-291), His Tag can bind to HEK293/Human Anti-CD19 Stable Cell Line. HEK293/Human Anti-CD19 Stable Cell Line was red line, Negative control HEK293 cells was grey line(QC tested).
Protocol
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背景
Chimeric antigen receptors (CARs) comprise interact domains, of which target cancer cell expressed antigens and allow T cell to efficiently kill the cancer cell. FMC63 is an IgG2a mouse monoclonal antibody specific for CD19, which is a target for the immunotherapy of B lineage leukaemias and lymphomas. FMC63 scFv is the most commonly used ectodomain component of CD19-specific CARs. Our CAR-like cells apply the HEK293 cells for the expression of FMC63 which will be presented on the cell membrane. It mimics the real CAR-T cell reaction and it is used as positive control for Anti-CD19 scFv Antibody flow cytometry detection.
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