SARS-CoV-2 Spike Trimer-coupled Magnetic Beads

背景（Background）

The pre-coupled magnetic beads coupled with biotinylated SARS-CoV-2 Spike trimer protein to streptavidin conjugated magnetic beads, which can capture the Anti- SARS-CoV-2 antibody or ACE2 protein from cell or serum sample. The SARS-CoV-2 Spike trimer protein, His Tag, Super stable trimer (SPN-C52H9) is the ectodomain of SARS-CoV-2 S protein which contains AA Val 16 - Pro 1213 (Accession # QHD43416.1). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively. The beads are in uniform size, narrow size distribution with large surface area and unique surface coating, which can help you get the best performance and highly reproducible results. This very first SARS-CoV-2 Spike trimer protein-coupled magnetic beads will bring great convenience with minimum non-specific binding and developed protocols. This ready to use products could greatly save your time and hassle.

表达区间及表达系统（Source）

SARS-CoV-2 Spike Trimer protein is expressed from human 293 cells (HEK293). It contains AA Val 16 - Pro 1213(Accession # QHD43416.1 (R683A, R685A)).

应用说明（Application）

This product is intended for immunocapture, biopanning. This is a non-sterile product.

重构方法（Reconstitution）

See Certificate of Analysis (CoA) for detailed instruction.

存储（Storage）

Upon receipt, please store the Beads at -20°C for 1 year in lyophilized state.

Please avoid more than 3 freeze-thaw cycles once reconstitution, immediate use after reconstitution is highly recommended.

原理（Assay Principles）

Antibody Purification: 1. Resuspend the lyophilized beads by adding the buffer of choice. 2. Add analyte to the suspension, mix and incubate to enable specific binding of the beads and the target protein. 3. Magnetize beads, remove supernatant, and wash unbound protein fractions to capture target protein-bound beads. 4. Wash, magnetize the beads and collect purified target protein for use in downstream applications.

The magnetic beads technology makes use of the easy and efficient collection of beads in magnetic field to facilitate antibody purification in a simple workflow of “bind-wash-elute”. In contrast to common separation techniques, this method does not require columns or centrifugation, and is therefore ideal in high-throughput applications.

制剂（Formulation）

Please contact us for detailed information.

Contact us for customized product form or formulation.