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Anti-SARS-CoV-2 Spike S1 Antibody, Mouse IgG1 (AS59)

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S1N-S59A1-100ug
¥3990.00
S1N-S59A1-1mg
¥26250.00
合计0件 产品金额¥ 0

产品详情

  • 抗体来源(Source)

    Anti-SARS-CoV-2 Spike S1 Antibody, Mouse IgG1 (AS59) (S1N-S59A1), originally from mouse immunized with recombinant SARS-CoV-2 Spike S1 protein, is produced from hybridoma.

  • 克隆号(Clone)

    AS59

  • 种属(Species)

    Mouse

  • 亚型(Isotype)

    Mouse IgG1 | Kappa

  • 偶联(Conjugate)

    Unconjugated

  • 抗体类型(Antibody Type)

    Hybridoma Monoclonal

  • 种属反应性(Reactivity)

    Virus

  • 特异性(Specificity)

    This product is a specific antibody against SARS-CoV-2 Spike protein RBD domain. No cross-reactivity is detected with Spike protein RBD domain of other coronaviruses, including SARS-CoV, MERS-CoV, HCoV-229E, HCoV-NL63, HCoV-OC43 and HCoV-HKU1.

  • 应用(Application)

    Application
    Recommended Usage
    ELISA
    0.1-10 ng/mL
  • 纯度(Purity)

    95% as determined by SDS-PAGE.

  • 纯化(Purification)

    Protein A purified / Protein G purified

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

数据展示

  • 电泳(SDS-PAGE)

    Spike S1 SDS-PAGE

    Anti-SARS-CoV-2 Spike S1 Antibody, Mouse IgG1 (AS59) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • 活性(Bioactivity)-ELISA

     Spike S1 ELISA

    Immobilized SARS-CoV-2 S protein RBD, His Tag (Cat. No. SPD-C52H1) at 2 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike S1 Antibody, Mouse IgG1 (AS59) (Cat. No. S1N-S59A1) with a linear range of 0.08-3 ng/mL (QC tested).

    Protocol

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背景介绍

It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

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