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分子别名（Synonym）
CD28,Tp44
表达区间及表达系统（Source）
Biotinylated Human / Cynomolgus / Rhesus macaque CD28, Fc,Avitag (CD8-H82F2) is expressed from human 293 cells (HEK293). It contains AA Asn 19 - Pro 152 (Accession # AAH93698). In the region Asn 19 - Pro 152, the AA sequence of Human, Cynomolgus and Rhesus macaque CD28 are homologus.
Predicted N-terminus: Asn 19
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 43.6 kDa. The protein migrates as 55-70 kDa under reducing (R) condition, and 110-130 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
产品数据图
电泳（SDS-PAGE）
Biotinylated Human / Cynomolgus / Rhesus macaque CD28, Fc,Avitag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Biotinylated Human / Cynomolgus / Rhesus macaque CD28, Fc,Avitag (Cat. No. CD8-H82F2) is more than 90% and the molecular weight of this protein is around 133-163 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Biotinylated Human / Cynomolgus / Rhesus macaque CD28, Fc,Avitag (Cat. No. CD8-H82F2) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Human B7-1, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. B71-H52A4) with a linear range of 5-78 ng/mL (QC tested).
Protocol
Immobilized Anti-CD28 MAb, Mouse IgG1 at 5 μg/mL (100 μL/well) can bind Biotinylated Human / Cynomolgus / Rhesus macaque CD28, Fc,Avitag (Cat. No. CD8-H82F2) with a linear range of 2.4-78 ng/mL (Routinely tested).
Protocol
活性（Bioactivity）-BLI
Loaded Biotinylated Human / Cynomolgus / Rhesus macaque CD28, Fc,Avitag (Cat. No. CD8-H82F2) on SA Biosensor, can bind Human B7-1, Fc Tag (Cat. No. B71-H5259) with an affinity constant of 56.8 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Biotinylated Human / Cynomolgus / Rhesus macaque CD28, Fc,Avitag (Cat. No. CD8-H82F2) on SA Biosensor, can bind Human B7-1, Mouse IgG2a Fc Tag (Cat. No. B71-H52A4) with an affinity constant of 38.3 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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背景
T-cell-specific surface glycoprotein CD28 is also known as TP44, is a single-pass type I membrane protein which contains one Ig-like V-type (immunoglobulin-like) domain. is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.
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