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组分（Materials Provided）
| ID | Components | Size |
| ADC-P009-C01 | 20 x Reaction buffer | 100ul |
| ADC-P009-C02 | Cofactor A | 50ul |
| ADC-P009-C03 | Cofactor B | 50ul |
| ADC-P009-C04 | Substrate A | 120ul |
| ADC-P009-C05 | Substrate B | 120ul |
| ADC-P009-C06 | Enzyme A | 120ul |
| ADC-P009-C07 | Enzyme B | 120ul |
| ADC-P009-C08 | Enzyme C | 40ul |
| ID | Components | Size |
| ADC-P009-1-C01 | Binding buffer | 15ml |
| ADC-P009-1-C02 | Elution buffer | 2ml×2 |
| ADC-P009-1-C03 | Neutralization buffer | 1ml |
| ADC-P009-1-C04 | 250 mM Tris-HCl buffer (pH7.5) | 1.5ml |
| ADC-P009-1-C05 | 10×PBS (pH7.2- 7.4) | 6ml |
| ADC-P009-1-C06 | Desalting column | 2per |
| ADC-P009-1-C07 | Concentrator tube | 2per |
| ADC-P009-1-C08 | ProteinA Resin | 100ul×5 |
产品数据图
典型数据-Typical Data Please refer to DS document for the assay protocol.
ADC DAR value assessment by RP-HPLC
DAR (Drug Antibody Ratio) value of ADC product was analyzed by RP-HPLC (QC tested).
Column: TSK gel Butyl-NPR column (4.6 mm × 35 mm, 2.5μm; TOSOH)
Mobile phase A：20 mM sodium phosphate, 1.5 M ammonium sulfate (pH 6.9)
Mobile phase B：75% (v/v) 20 mM sodium phosphate, 25% (v/v) isopropanol (pH 6.9)
Flow rate: 0.4 mL/min
Column temperature: 30 °C Detection wavelength：280nm
Loading Amount:10 µg (5-10 µL)
DAR (Drug Antibody Ratio) value of ADC product was analyzed by RP-HPLC (QC tested).
DAR4 =(0*1.5+1*4.38+2*2.25+3*7+4*84.88)/100=3.694
DAR2＝(0*0.52+2*99.48)/100=1.9896
ADC DAR value assessment by RP-HPLC
DAR (Drug Antibody Ratio) value of ADC product was analyzed by RP-HPLC (QC tested).
Column: TSK gel Butyl-NPR column (4.6 mm × 35 mm, 2.5μm; TOSOH)
Mobile phase A：20 mM sodium phosphate, 1.5 M ammonium sulfate (pH 6.9)
Mobile phase B：75% (v/v) 20 mM sodium phosphate, 25% (v/v) isopropanol (pH 6.9)
Flow rate: 0.4 mL/min
Column temperature: 30 °C Detection wavelength：280nm
Loading Amount:10 µg (5-10 µL)
DAR (Drug Antibody Ratio) value of ADC product was analyzed by RP-HPLC (QC tested).
DAR4 =(0*1.5+1*4.38+2*2.25+3*7+4*84.88)/100=3.694
DAR2＝(0*0.52+2*99.48)/100=1.9896
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