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应用（Application）
Flow Cytometry (Evaluation of the expression of CD45 on Human cells).
种属（Species）
Mouse
亚型（Isotype）
Mouse IgG2a
特异性（Specificity）
This product is a specific antibody specifically reacts with CD45 protein.
种属反应性（Reactivity）
Human
偶联（Conjugate）
PerCP-Cy5.5
Excitation Wavelength: 488 nm
Emission Wavelength: 690 nm
推荐稀释比（Recommended Dilution）
1:20
制剂（Formulation）
Supplied as 0.2 μm filtered solution in PBS, 0.2% BSA, 0.03% Proclin 300, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
存储（Storage）
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- Store at 2-8 °C for 12 months.
产品数据图
活性（Bioactivity）-FACS
Flow cytometric analysis of Human peripheral blood lymphocytes respectively staining with PerCP-CY5.5-Labeled Anti-Human CD45 Antibody, mouse IgG2a (AIRFS225-240625F1-Bulk) at 1:20 dilution (5 μL of the antibody stock solution corresponds to labeling of 2.5e5 cells in a final volume of 100 µL), compared with isotype control antibody. PerCP-CY5.5 signal was used to evaluate the binding activity (QC tested).
Protocol
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背景
CD45 is a receptor protein tyrosine phosphatase, also known as Ly-5 or leukocyte common antigen. CD45 mainly involves in the initiation of T cell receptor signaling by controlling the activation of the Src family protein-tyrosine kinases Lck and Fyn. CD45 deficiency causes in T- and B-lymphocyte dysfunction in the form of severe combined immune deficiency. It also takes a significant role in autoimmune diseases and cancer as well as in infectious diseases including fungal infections.
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