CD19蛋白-专为检测CD19-CAR-T而设计
CD19是正常和恶性B淋巴细胞特异性表面蛋白，在B细胞的发育、增殖和分化以及恶性转化中发挥重要作用。因CD19在B淋巴细胞表达的特异性和恶性肿瘤表达的广泛性，使其成为一个颇具潜力的B淋巴细胞恶性肿瘤免疫治疗的分子靶点。靶向CD19的热门免疫治疗策略包括嵌合抗原受体修饰T细胞（CAR-T）、双特异性抗体、抗体-药物偶联物（ADC）、Fc优化免疫增强抗体等，均在B细胞淋巴瘤治疗方面表现出了显著的疗效。截至2020年5月7日，已上市药物有3款，处于临床研究阶段项目763项（数据来自ClinicalTrials.gov）。
为支持靶向CD19的免疫治疗药物的开发，ACROBiosystems专门设计开发了一系列适用于免疫、抗体药筛选、亲和力检测及Anti-CD19 CAR表达检测的高亲和力CD19蛋白，包括非标记、生物素标记、荧光标记等多种形式，通过Flow cytometry、SPR、ELISA等检测方法对其性能进行了全方位的分析，保证蛋白纯度和活性，助力您的药物研发进程。
ACRO更有FDA DMF备案的CD19蛋白（DMF编号：034936）支持您的IND、NDA和BLA申请，如果您使用了DMF备案CD19蛋白并需要引用DMF编号，请向ACRO提出授权申请，收到申请后，我们将向FDA提供DMF授权书。点击申请授权
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经流式验证适用于Anti-CD19 CAR阳性表达率检测
- FACS Analysis of Anti-CD19 CAR Expression
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- 5e5 of anti-CD19 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of PE-Labeled Human CD19 (20-291), His Tag (Cat. No. CD9-HP2H5) and negative control protein respectively (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). PE signal was used to evaluate the binding activity (QC tested).
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Protocol
高亲和力经SPR验证
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- Biotinylated Human CD19 (20-291), His,Avitag (Cat. No. CD9-H82E9) captured on Biotin CAP - Series S sensor Chip can bind FMC63 MAb (mouse lgG2a) with an affinity constant of 0.255 nM as determined in a SPR assay (Biacore 8K) (QC tested).
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Protocol
经ELISA、FACS 和 SPR验证活性显著高于竞品
Binding activity of FITC-Labeled Human CD19, His Tag from two different vendors were evaluated in the ELISA analysis against FMC63 MAb above. The result showed that ACRO's FITC-Labeled Human CD19, His Tag has a much higher binding activity than that of the other vendor.
Report
Binding activity of FITC-Labeled Human CD19, His Tag from two different vendors were evaluated in the flow cytometry analysis against anti-CD19-CAR-293 cells above. The result showed that ACRO's FITC-Labeled Human CD19, His Tag has a much higher binding activity than that of the other vendor.
Report
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- Binding activity of Human CD19, His Tag from two different vendors were evaluated by SPR assay against FMC63 MAb. The result showed that ACRO's Human CD19, His Tag can bind FMC63 MAb with an affinity constant of 2.95 nM which is much higher than that of the other vendor.
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Report
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