收藏产品 产品信息
表达区间及表达系统（Source）
Biotinylated Human respiratory syncytial virus A (strain A2) Pre-F0, His,Avitag (RSF-V82E7) is expressed from human 293 cells (HEK293).
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 57.8 kDa. The protein migrates as 60-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 0.1 M Sodium citrate, 150 mM NaCl, pH5.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
产品数据图
电泳（SDS-PAGE）
Biotinylated Human respiratory syncytial virus A (strain A2) Pre-F0, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Human respiratory syncytial virus A (strain A2) Pre-F0, His,Avitag (Cat. No. RSF-V82E7) is more than 85% and the molecular weight of this protein is around 185-225 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Anti-Fusion glycoprotein F0 Antibody, Human IgG1 (D25) at 5 μg/mL (100 μL/well) can bind Biotinylated Human respiratory syncytial virus A (strain A2) Pre-F0, His,Avitag (Cat. No. RSF-V82E7) with a linear range of 0.1-8 ng/mL (QC tested).
Protocol
Immobilized Anti-Fusion glycoprotein F0 Antibody, Mouse IgG2a (101F) at 5 μg/mL (100 μL/well) can bind Biotinylated Human respiratory syncytial virus A (strain A2) Pre-F0, His,Avitag (Cat. No. RSF-V82E7) with a linear range of 0.2-16 ng/mL (QC tested).
Protocol
Immobilized Recombinant Mouse Anti-RSV F Antibody (5C4) at 5 μg/mL (100 μL/well) can bind Biotinylated Human respiratory syncytial virus A (strain A2) Pre-F0, His,Avitag (Cat. No. RSF-V82E7) with a linear range of 0.2-16 ng/mL (QC tested).
Protocol
产品评论 发表评论
背景
Human respiratory syncytial virus (HRSV) is the most common etiological agent of acute lower respiratory tract disease in infants and can cause repeated infections throughout life. The RSV fusion glycoprotein (RSV F) is the principal target of RSV neutralizing antibodies in human sera. The RSV F is a type I viral fusion protein synthesized as inactive, single-chain polypeptides that assemble into trimers. RSV F fuses the viral and host cell membranes by irreversible protein refolding from the labile prefusion conformation to the stable post-fusion conformation.
数据表和文档 
联系我们 
项目合作