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分子别名（Synonym）
Coagulation Factor III,Tissue Factor,TF,F3,CD142
表达区间及表达系统（Source）
Human Tissue Factor, His Tag (TF3-H52H5) is expressed from human 293 cells (HEK293). It contains AA Ser 33 - Glu 251 (Accession # P13726-1).
Predicted N-terminus: Ser 33
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 26.7 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
产品数据图
电泳（SDS-PAGE）
Human Tissue Factor, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Human Tissue Factor, His Tag (Cat. No. TF3-H52H5) is more than 90% and the molecular weight of this protein is around 30-40 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human Tissue Factor, His Tag (Cat. No. TF3-H52H5) at 1 μg/mL (100 μL/well) can bind Anti-TF Antibody, Human IgG1 with a linear range of 0.2-2 ng/mL (Routinely tested).
Protocol
活性（Bioactivity）-SPR
Anti-TF mAb immobilized on CM5 Chip via anti-human IgG Fc antibody can bind Human Tissue Factor, His Tag (Cat. No. TF3-H52H5) with an affinity constant of 1.52 nM as determined in SPR assay (Biacore T200) (Routinely tested).
Protocol
活性（Bioactivity）
Measured by its ability to activate Coagulation Factor VII in cleaving a fluorogenic peptide substrate Boc-VPR-AMC. The AC50 is <4.0 μg/mL, as measured under the described conditions (QC tested).
Protocol
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背景
Full-length tissue factor (TF) is a transmembrane receptor and cofactor for factor (F)VII/FVIIa. In addition to full-length TF, an alternative spliced (as) form of TF can be generated that lacks the transmembrane domain and is released fromcells. In contrast to TF, asTF has low procoagulant activity because it lacks the transmembrane domain. Tissue factor is expressed by cells around blood vessels, such as adventitial fibroblasts, and body surfaces, such as epithelial cells, and plays a critical role in hemostasis. TF also contributes to various forms of thrombosis. Many cancers, particularly adenocarcinomas, express high levels of TF. A high level of tumor TF expression is associated with poor prognosis in many types of cancers, including breast, prostate, colorectal, and pancreatic cancer.
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