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分子别名（Synonym）
CD8 alpha & beta,CD8A & CD8B
表达区间及表达系统（Source）
FITC-Labeled Human CD8A&CD8B Heterodimer Protein, His Tag&Tag Free (CDA-HF2W4) is expressed from human 293 cells (HEK293). It contains AA Ser 22 - Asp 182 (CD8A) & Leu 22 - Pro 170 (CD8B) (Accession # P01732-1 (CD8A) & P10966-1 (CD8B)). It is the FITC labeled form of Human CD8A&CD8B Heterodimer Protein, His Tag&Tag Free (CDA-H52W5).
Predicted N-terminus: Ser 22 (CD8A) & Leu 22 (CD8B)
Request for sequence
蛋白结构（Molecular Characterization）
FITC-Labeled Human CD8A&CD8B Heterodimer Protein, His Tag&Tag Free, produced by co-expression of CD8 alpha and CD8 beta, has a calculated MW of 19.5 kDa (CD8 alpha) and 22.1 kDa (CD8 beta). Subunit CD8 alpha is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit CD8 beta contains no tag but a basic tail at the C-terminus. As a result of glycosylation, the heterodimer protein migrates as 30-32 kDa and 32-34 kDa under reducing (R) condition, and 30-33 kDa and 60-65 kDa under non-reducing (NR) condition (SDS-PAGE).
偶联（Conjugate）
FITC
Excitation source: 488 nm spectral line, argon-ion laser
Excitation Wavelength: 488 nm
Emission Wavelength: 535 nm
标记（Labeling）
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.
蛋白标记度（Protein Ratio）
The FITC to protein molar ratio is 2-3.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
产品数据图
电泳（SDS-PAGE）
FITC-Labeled Human CD8A&CD8B Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
活性（Bioactivity）-ELISA
Immobilized Anti-CD8 alpha Antibody, Mouse IgG2a (clone: OKT8) at 1 μg/mL (100 μL/well) can bind FITC-Labeled Human CD8A&CD8B Heterodimer Protein, His Tag&Tag Free (Cat. No. CDA-HF2W4) with a linear range of 0.625-2.5 μg/mL (QC tested).
Protocol
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背景
Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. CD8A forms disulfide-linked heterodimers with CD8B at the cell surface. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells. Additionally, CD8B plays a critical role in thymic selection of CD8+ T-cells. A palmitoylation site in the cytoplasmic tail of CD8B chain contributes to partitioning of CD8 into the plasma membrane lipid rafts where signaling proteins are enriched.
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