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分子别名（Synonym）
IAP
表达区间及表达系统（Source）
Human ALPI Protein, His Tag (ALI-H52H3) is expressed from human 293 cells (HEK293). It contains AA Val 20 - Asp 503 (Accession # P09923-1).
Predicted N-terminus: Val 20
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 54.3 kDa. The protein migrates as 60-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Supplied as 0.2 μm filtered solution in 20 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
产品数据图
电泳（SDS-PAGE）
Human ALPI Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human ALPI Protein, His Tag (Cat. No. ALI-H52H3) is more than 90% and the molecular weight of this protein is around 100-130 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human ALPI Protein, His Tag (Cat. No. ALI-H52H3) at 1 μg/mL (100 μL/well) can bind various dilution ratio of Anti-ALPI Polyclonal Antibody (QC tested).
Protocol
活性（Bioactivity）
Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP). The specific activity is >800 pmol/min/μg (QC tested).
Protocol
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背景
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is a component of the gut mucosal defense system and is thought to function in the detoxification of lipopolysaccharide, and in the prevention of bacterial translocation in the gut.
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