收藏产品 产品信息
- Genetically modified cell lines best reflect MOA (Mechanism of Action)
- Higher activity and larger assay window for robust and reproducible cell-based bioassay
- Comprehensive application data to support assay development and validation
- Full tracible record, stringent quality control and validated cell passage stability
- Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
- Global commercial license assistance whenever regulatory filing is required
描述（Description）
The NFAT (Luc) HEK293 Reporter Cell was engineered with the NFAT response element driving luciferase expressing systems. The receptors expressing endogenously or transfected on this reporter cell were activated by corresponding ligands binding, transducing intracellular signals resulting in NFAT-RE mediated luminescence.
应用说明（Application）
• The discovery of activators or inhibitors by the NFAT signaling bioactivity
• Transfection host for some receptors concerning the NFAT signaling pathway
生长特性（Growth Properties）
Adherent
筛选标记（Selection Marker）
Hygromycin B (50 μg/mL)
培养基（Complete Growth Medium）
DMEM medium + 10% FBS
冻存液（Freeze Medium）
Serum-free cell cryopreservation medium
装量（Quantity）
1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
存储（Storage）
Frozen in liquid nitrogen.
支原体检测（Mycoplasma Testing）
Negative
无菌检测（Sterility Testing）
Negative
使用说明（Instructions for Use）
See data sheet for detailed culturing and assay protocol.
产品数据图
Signaling Bioassay
Response to PMA plus Ionomycin (RLU).
The NFAT (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of PMA plus Ionomycin (2 μM). The EC50 was approximately 3.4 ng/mL.
Protocol
Response to PMA plus Ionomycin (Fold).
The NFAT (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of PMA plus Ionomycin (2 μM). The max induction fold was approximately 430.
Protocol
Passage Stability
Passage stability analysis by Signaling Bioassay.
The continuously growing NFAT (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of PMA plus Ionomycin (2 μM). PMA plus Ionomycin stimulated response demonstrates passage stabilization (fold induction and EC50) across passage 25-35. And, the bioactivity of NFAT signaling still can be detected by PMA plus Ionomycin stimulation on passage 35 with high activating fold.
Protocol
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背景
Nuclear factor of activated T cells (NFAT), which is the pharmacological target of immunosuppressants cyclosporine and tacrolimus, has been shown to play an important role in T cells (immune system) and many biological events. Accumulating studies have indicated that NFATs are involved in many aspects of cancer, including carcinogenesis, cancer cell proliferation, invasion, metastasis, angiogenesis, drug resistance and tumor microenvironment. As the key section of the adaptive immune response, the T cell receptor (TCR)-calcium-calcineurin signaling pathway could lead to T cell activation. The 'nuclear factor of activated T cells' proteins are transcription factors that promote expression of a panel of genes required for activation.
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