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抗体来源（Source）
Monoclonal Anti-His Tag Antibody, Human IgG1 (AY63) is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain.
克隆号（Clone）
AY63
亚型（Isotype）
Human IgG1 | Human Kappa
偶联（Conjugate）
Unconjugated
抗体类型（Antibody Type）
Recombinant Monoclonal
种属反应性（Reactivity）
Tag
免疫原（Immunogen）
A protein peptide with his tag.
特异性（Specificity）
This product is a specific antibody specifically reacts with His tag protein, and can recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.
应用（Application）
| Application | Recommended Usage |
| Western Blot | 0.2-1 ug/mL |
| ELISA | 0.1-16 ng/mL |
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
纯化（Purification）
Protein A purified / Protein G purified
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
产品数据图
电泳（SDS-PAGE）
Monoclonal Anti-His Tag Antibody, Human IgG1 (AY63) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Monoclonal Anti-His Tag Antibody, Human IgG1 (AY63) (Cat. No. HIS-M535) is more than 90% and the molecular weight of this protein is around 135-155 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Canine TNF-alpha, His Tag (Cat. No. TNA-C52H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-His Tag Antibody, Human IgG1(AY63) (Cat. No. HIS-M535) with a linear range of 0.1-1 ng/mL (QC tested).
Protocol
活性（Bioactivity）-SPR
Monoclonal Anti-His Tag Antibody, Human IgG1 (AY63) (Cat. No. HIS-M535) captured on Protein G Chip can bind Rabbit Serum Albumin, His Tag (Cat. No. RSA-R52H6) with an affinity constant of 29.6 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Western Blot验证
Detection of Monoclonal Anti-His Tag Antibody, Human IgG1 (AY63), mAb by Western Blot. Monoclonal Anti-His Tag Antibody, Human IgG1 (AY63), mAb at 1/20000 dilution + Human LAG-3 / CD223 Protein , His Tag at 75-250ng.
Secondary Antibody: (HFC)-HRP Goat Anti-Human IgG,Fcγ fragment specific (min X Bov,Hrs,Ms Sr Prot) at 1/2000 dilution.
Predicted band size: 60 kDa 12% Bis-Tris Protein Gel.
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背景
His tag is a very small molecular weight tag, usually composed of 6-10 histidine (His). It is one of the commonly used tags for protein purification and detection. Due to its small molecular weight, fusion into the target protein has almost no effect on the structure and characteristics of the protein. Anti-his tag antibody can accurately detect, locate and purify His tag fusion protein, so as to provide convenience for the vast number of researchers.
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